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Uropatogenne Szczepy Escherichia Coli

Uropatogenne Szczepy Escherichia Coli


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About the Book

Zakażenia ukladu moczowego są jedną z najczęstszych przyczyn zglaszania się chorych do lekarza. Ponad 80% wszystkich zakażeń wywolywanych jest przez Escherichia coli, dlatego bakteria ta jest obiektem wielu badań związanych z tematyką zakażeń ukladu moczowego. Przedmiotem pracy bylo zbadanie puli 111 izolatów E. coli pochodzących od 29 pacjentów Gdańskiego Uniwersytetu Medycznego z Kliniki Nefrologii, Transplantologii i Chorób Wewnętrznych oraz Poradni Nefrologicznej. Szczepy izolowane byly z krwi oraz moczu. Do badań przeznaczono szczepy, które różnily się morfologicznie w hodowlach na plytkach. Izolaty te badano pod kątem identyczności genetycznej stosując metodę genotypowania PCR-MP (ang. Melting Profiles). Celem tych badań bylo stwierdzenie, bądź też wykluczenie transmisji bakterii z ukladu moczowego do lożyska krwionośnego. Ponadto wykrywano siedem czynników wirulencji takich jak: fimbrie typu: P, S, 1, Dr, cytotoksyczny czynnik nekrotyzujący, bakteriocynę Usp oraz α hemolizynę odpowiedzialnych za patogennośc drobnoustrojów. Do wykrywania tych czynników wirulencji wykorzystano reakcję PCR. Otrzymane wyniki pozwolily określic czy dochodzilo do transmisji bakterii z ukladu moczowego do krwi, a także wskazaly glówne czynniki wirulencji posiadane przez uropatogenne szczepy Escherichia coli, które zdolne byly do wywolania bakteriemii wśród badanej puli pacjentów.


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Product Details
  • ISBN-13: 9783639890419
  • Publisher: Wydawnictwo Bezkresy Wiedzy
  • Binding: Paperback
  • Language: Polish
  • Returnable: N
  • Spine Width: 5 mm
  • Width: 152 mm
  • ISBN-10: 3639890418
  • Publisher Date: 24 Apr 2014
  • Height: 229 mm
  • No of Pages: 76
  • Series Title: Polish
  • Weight: 172 gr


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