Part I. Synthesis of Unnatural Amino Acids

1. Preparation of Unnatural Amino Acids with Ammonia-lyases and 2,3-aminomutases

László Poppe, Csaba Paizs, Klaudia Kovács, Florin-Dan Irimie, and Beáta Vértessy

2. Multistep Enzyme Catalyzed Reactions for Unnatural Amino Acids

Paola D'Arrigo and Davide Tessaro

3. Enzymatic Production of Enantiopure Amino Acids from Mono-substituted Hydantoin Substrates

Gwynneth F. Matcher, Rosemary A. Dorrington, and Stephanie G. Burton

4. Preparation of Glutamate Analogues by Enzymatic Transamination

Thierry Gefflaut, Zeinab Assaf, and Martine Sancelme

5. Carbon-Carbon Bond Forming Enzymes for the Synthesis of Non-natural Amino Acids

Pere Clapés, Jesús Joglar, and Mariana Gutiérrez

6. Engineering Cyclic Amidases for Non-natural Amino Acid Synthesis

Francisco Javier Las Heras-Vázquez, Josefa María Clemente-Jiménez, Sergio Martínez-Rodríguez, and Felipe Rodríguez-Vico

Part II. Application of Unnatural Amino Acids

7. NMR Analysis of Unnatural Amino Acids in Natural Antibiotics

Franca Castiglione

8. Site-specific Incorporation of Unnatural Amino Acids as Probes for Protein Conformational Changes

Jennifer C. Peeler and Ryan A. Mehl

9. Application of Unnatural Amino Acids to the De novo Design of Selective Antibiotic Peptides

Rickey P. Hicks and Amanda L. Russell

10. Use of Unnatural Amino Acids to Probe Structure-activity Relationships and Mode-of-action of Antimicrobial Peptides

Alessandro Tossi, Marco Scocchi, Sotir Zahariev, and Renato Gennaro

Part III. Use of Unnatural Amino Acids in Protein Synthesis

11. Experimental Methods for Scanning Unnatural Amino Acid Mutagenesis

Jia Liu and Ashton T. Cropp

12. Genetic Incorporation of Unnatural Amino Acids into Proteins in Yeast

Qian Wang and Lei Wang

13. Site-specific Incorporation of Unnatural Amino Acids into Proteins in Mammalian Cells

Nobumasa Hino, Kensaku Sakamoto, and Shigeyuki Yokoyama

14. Incorporation of Unnatural Non-a-amino Acids into the N-terminus of Proteins in a Cell-free Translation System

Takahiro Hohsaka

15. Site Specific Modification of Proteins by the Staudinger-phosphite Reaction

Paul Majkut, Verena Böhrsch, Remigiusz Serwa, Michael Gerrits, and Christian P. R. Hackenberger

Part IV. D-amino Acids: Analysis and Applications

16. HPLC Methods for Determination of D-aspartate and N-methyl-D-aspartate

George H. Fisher and Mara Tsesarskaia

17. Estimation of Chronological Age from the Racemization Rate of L- and D-aspartic Acid: How to Completely Separate Enantiomers from Dentin

Toshiharu Yamamoto and Susumu Ohtani

18. Enzymatic Detection of D-amino Acids

Gianluca Molla, Luciano Piubelli, Federica Volontè, and Mirella S. Pilone

19. An Enzymatic-HPLC Assay to Monitor Endogenous D-serine Release from Neuronal Cultures

Inna Radzishevsky and Herman Wolosker

20. Electrophysiological Analysis of the Modulation of NMDA-receptors Function by d-serine and Glycine in the Central Nervous System

Fabrice Turpin, Glenn Dallérac, and Jean-Pierre Mothet

21. Biosensors for D-amino Acid Detection

Silvia Sacchi, Elena Rosini, Laura Caldinelli, and Loredano Pollegioni

22. Analysis of D-β-aspartyl Isomers at Specific Sites in Proteins

Noriko Fujii and Norihiko Fujii

23. N