A Study in Scarlet is a detective novel by British author Arthur Conan Doyle. Written in 1886, the story marks the first appearance of Sherlock Holmes and Dr. Watson, who would become two of the most famous characters in popular fiction. The book's title derives from a speech given by Holmes, an amateur detective, to his friend and chronicler Watson on the nature of his work, in which he describes the story's murder investigation as his "study in scarlet": "There's the scarlet thread of murder running through the colourless skein of life, and our duty is to unravel it, and isolate it, and expose every inch of it." The story, and its main characters, attracted little public interest when it first appeared. Only 11 complete copies of the magazine in which the story first appeared, Beeton's Christmas Annual for 1887, are known to exist now and they have considerable value. Although Conan Doyle wrote 56 short stories featuring Holmes, A Study in Scarlet is one of only four full-length novels in the original canon. The novel was followed by The Sign of the Four, published in 1890. A Study in Scarlet was the first work of detective fiction to incorporate the magnifying glass as an investigative tool. Part I leads with a heading which establishes the role of Dr. Watson as narrator and sets up the narrative stand-point that the work to follow is not fiction, but fact: "Being a Reprint from the Reminiscences of John H. Watson, MD, Late of the Army Medical Department." The story begins in 1881, when Dr. Watson, having returned to London after serving in the Second Anglo-Afghan War, runs into an old friend named Stamford at the Criterion Restaurant, who had been a dresser under him at St. Bartholomew's Hospital . Watson confides in Stamford that, due to a shoulder injury that he sustained at the Battle of Maiwand, he has been forced to leave the armed services and is now looking for a place to live. Stamford mentions that an acquaintance of his, Sherlock Holmes, is looking for someone to split the rent at a flat at 221B Baker Street, but he cautions Watson about Holmes's eccentricities. Stamford takes Watson back to St. Bartholomew's where, in a laboratory, they find Holmes experimenting with a reagent, seeking a test to detect human haemoglobin. Holmes explains the significance of bloodstains as evidence in criminal trials. After Stamford introduces Watson to Holmes, Holmes shakes Watson's hand and comments, "You have been in Afghanistan, I perceive?" Though Holmes chooses not to explain why he made the comment, Watson raises the subject of their parallel quests for a place to live in London, and Holmes explains that he has found the perfect place in Baker Street. At Holmes's prompting, the two review their various shortcomings to make sure that they can live together. After seeing the rooms at 221B, they move in and grow accustomed to their new situation.