full screen
Home > Science & Mathematics > Biology, life sciences > Life sciences: general issues > Neurosciences > Site-Directed Insertion of Transgenes
Site-Directed Insertion of Transgenes

Site-Directed Insertion of Transgenes


     0     
5
4
3
2
1



International Edition


About the Book

Preface.- Section 1: Site-directed integration of transgenes.- 1 Transgene site-specific integration: troubles and solutions.- 1.1 Introduction.- 1.2 Random integration and clonal dominance: reality or myth.- 1.3 Principal drawbacks of gene transfer integrative systems and solutions.- 1.4 Combining long-term expression and secure integration by specific locus targeting: old solutions to new perspectives.- 1.5 Conclusion.- 2: Designing non-viral targetd integrating vectors for genome enginnering in vertebrates.- 2.1. Introduction: Ideal integrating vectors and safe insertion sites.- 2.2. Design of random integrating vectors based on DNA transposons and associated-targeting strategy.- 2. 3. Site-specific recombinase-based integrating vectors.- 2. 4. Meganuclease-based vectors.- Section 2: Integration based on homologous recombination.- 3: Gene targeting and homologous recombination in Saccharomyces cerevisiae.- 3.1 Introduction.- 3.2 Transformation with linearized plasmids: targeted integration.- 3.3 Mechanism of targeted integration: a state of the art of homologous recombination.- 3.4 Conclusions and future directions.- 4: Eucaryotic homologous recombination in mammalian cells.- 4.1. Models and products of HR.- 4.2. Molecular mechanisms of the different steps of HR.- 4.3. HR is at the heart of the genetic stability-diversity-instability equilibrium. - 4.4. The importance of sister chromatids in the cell cycle for the maintenance of genome stability.- 4.5. Deregulation of HR and tumor predisposition.- 4.6. HR in molecular evolution: concerted evolution.- 4.7. Comments/considerations regarding optimization of targeted gene replacement. - 5 Engineered Zinc Finger Nucleases for Targeted Genome Editing .- 5.1 Introduction.- 5.2 Zinc Fingers: Structure and Function.- 5.3 Zinc Finger Fusion Proteins.- 5.4 Zinc Finger Engineering Platforms.- 5.5 Modified ZFN Architectures.- 5.6 Types of ZFN-Inducible Genome Modifications.- 5.7 Cell Line and Organismic Modifications using ZFNs.- 5.8 Off-Target Effects and Cytotoxicity.- 5.9 Conclusions.- 6: Engineered meganucleases for genome engineering purposes.- 6.1. Introduction.- 6.2. Historical considerations.- 6.3. The I-SceI success story.- 6.4. The challenge of specificity toward a chosen sequence.- 6.5. Cleavage versus Nickase activity.- 6.6. Customized meganucleases for genome engineering.- 6.7. Overview on TAL Effector Nuclease (TALEN).-6.8. Conclusions.- Section3: Integration based on Site-specific recombination.- 7: Cre/loxP, Flp/FRT systems and pluripotent stem cell lines.- 7.1. A historical perspective on Cre/loxP and Flp/FRT systems.- 7.2. Mechanisms of Cre/loxP and Flp/FRT recombination.- 7.3. Mutant lox and FRT sites.- 7.4. Pluripotent stem cells, an overview.- 7.5. Basic strategies for site-specific integration of transgenes via Cre/loxP and Flp/FRT systems.- 7.6 Delivery of recombinases into PSCs.- 7.7. Applications of Cre/loxP and Flp/FRT systems in the introduction of transgenes to PSCs.- 7.8. Technological improvements and future outlook.- 8: Site-specific recombination using C31 Integrase.- 8.1 Introduction.- 8.2 C31 Integrase: Mechanism of Integration and Optimization for Heterologous Organisms.- 8.3 Use of C31 in Heterologous Organisms.- 8.4 Therapeutic Uses of C31 Integrase.- 8.5 Use of C31 in Pluripotent Stem Cells 24.- 8.6 Safety of the C31 Integrase System 28.- 8.7 The Future of C31 Integrase: Combinatorial Usage With Other Recombinases.- 9: Modified transposases used for site-directed insertion of transgenes.- 9. 1 Introduction.- 9.2 Targeted transposition in bacteria.- 9.3 Targeted transposition in insects.- 9.4 Targeted transposition in zebrafish .- 9.5 Targeted transposition in mammalian cells.- 9.6 Conclusion-Discussion.- 10: Targeted Plasmid Integration into the Human Genome by Engineered Recombinases.- 10.1 Introduction.- 10.2 Nonviral methods for transgene integration.- 10.3 Transgene integration by site-specific recombinases.- 10


Best Sellers



Product Details
  • ISBN-13: 9789401780759
  • Publisher: Springer
  • Publisher Imprint: Springer
  • Edition: 2013 ed.
  • Language: English
  • Returnable: N
  • Spine Width: 21 mm
  • Width: 156 mm
  • ISBN-10: 9401780757
  • Publisher Date: 09 Aug 2014
  • Binding: Paperback
  • Height: 234 mm
  • No of Pages: 394
  • Series Title: Topics in Current Genetics
  • Weight: 621 gr


Similar Products

Add Photo
Add Photo

Customer Reviews

REVIEWS      0     
Click Here To Be The First to Review this Product
Site-Directed Insertion of Transgenes
Springer -
Site-Directed Insertion of Transgenes
Writing guidlines
We want to publish your review, so please:
  • keep your review on the product. Review's that defame author's character will be rejected.
  • Keep your review focused on the product.
  • Avoid writing about customer service. contact us instead if you have issue requiring immediate attention.
  • Refrain from mentioning competitors or the specific price you paid for the product.
  • Do not include any personally identifiable information, such as full names.

Site-Directed Insertion of Transgenes

Required fields are marked with *

Review Title*
Review
    Add Photo Add up to 6 photos
    Would you recommend this product to a friend?
    Tag this Book Read more
    Does your review contain spoilers?
    What type of reader best describes you?
    I agree to the terms & conditions
    You may receive emails regarding this submission. Any emails will include the ability to opt-out of future communications.

    CUSTOMER RATINGS AND REVIEWS AND QUESTIONS AND ANSWERS TERMS OF USE

    These Terms of Use govern your conduct associated with the Customer Ratings and Reviews and/or Questions and Answers service offered by Booksbay (the "CRR Service").


    By submitting any content to Booksbay, you guarantee that:
    • You are the sole author and owner of the intellectual property rights in the content;
    • All "moral rights" that you may have in such content have been voluntarily waived by you;
    • All content that you post is accurate;
    • You are at least 13 years old;
    • Use of the content you supply does not violate these Terms of Use and will not cause injury to any person or entity.
    You further agree that you may not submit any content:
    • That is known by you to be false, inaccurate or misleading;
    • That infringes any third party's copyright, patent, trademark, trade secret or other proprietary rights or rights of publicity or privacy;
    • That violates any law, statute, ordinance or regulation (including, but not limited to, those governing, consumer protection, unfair competition, anti-discrimination or false advertising);
    • That is, or may reasonably be considered to be, defamatory, libelous, hateful, racially or religiously biased or offensive, unlawfully threatening or unlawfully harassing to any individual, partnership or corporation;
    • For which you were compensated or granted any consideration by any unapproved third party;
    • That includes any information that references other websites, addresses, email addresses, contact information or phone numbers;
    • That contains any computer viruses, worms or other potentially damaging computer programs or files.
    You agree to indemnify and hold Booksbay (and its officers, directors, agents, subsidiaries, joint ventures, employees and third-party service providers, including but not limited to Bazaarvoice, Inc.), harmless from all claims, demands, and damages (actual and consequential) of every kind and nature, known and unknown including reasonable attorneys' fees, arising out of a breach of your representations and warranties set forth above, or your violation of any law or the rights of a third party.


    For any content that you submit, you grant Booksbay a perpetual, irrevocable, royalty-free, transferable right and license to use, copy, modify, delete in its entirety, adapt, publish, translate, create derivative works from and/or sell, transfer, and/or distribute such content and/or incorporate such content into any form, medium or technology throughout the world without compensation to you. Additionally,  Booksbay may transfer or share any personal information that you submit with its third-party service providers, including but not limited to Bazaarvoice, Inc. in accordance with  Privacy Policy


    All content that you submit may be used at Booksbay's sole discretion. Booksbay reserves the right to change, condense, withhold publication, remove or delete any content on Booksbay's website that Booksbay deems, in its sole discretion, to violate the content guidelines or any other provision of these Terms of Use.  Booksbay does not guarantee that you will have any recourse through Booksbay to edit or delete any content you have submitted. Ratings and written comments are generally posted within two to four business days. However, Booksbay reserves the right to remove or to refuse to post any submission to the extent authorized by law. You acknowledge that you, not Booksbay, are responsible for the contents of your submission. None of the content that you submit shall be subject to any obligation of confidence on the part of Booksbay, its agents, subsidiaries, affiliates, partners or third party service providers (including but not limited to Bazaarvoice, Inc.)and their respective directors, officers and employees.

    Accept

    New Arrivals



    Inspired by your browsing history


    Your review has been submitted!

    You've already reviewed this product!