1. The Essential, Ubiquitous Single-Stranded DNA-binding Proteins
Marcos T. Oliveira and Grzegorz L. Ciesielski
2. Single-stranded DNA Binding Proteins in the Archaea
Najwa Taib, Simonetta Gribaldo, and Stuart A. MacNeill
3. Single-molecule Fluorescence Methods to Study Protein Exchange Kinetics in Supramolecular Complexes
Richard R. Spinks, Lisanne M. Spenkelink, and Antoine M. van Oijen
4. Comparing SSB-PriA Functional and Physical Interactions in Gram-positive and -Negative Bacteria
Yen-Hua Huang and Cheng-Yang Huang
5. In vivo binding of Single-stranded DNA-binding Protein to Stalled Replication Fork Helicases
Cong Yu and Piero R. Bianco
6. Magnetic Tweezers-based Single Molecule Assays to Study Interaction of E. coli SSB with DNA and RecQ Helicase
Debjani Bagchi, Weiting Zhang, Samar Hodeib, Bertrand Ducos, Vincent Croquette, and Maria Manosas
7. High-throughput Screening to Identify Inhibitors of SSB-Protein Interactions
Andrew F. Voter
8. Single-Molecule Tethered Particle Motion Studies on the DNA Recombinase Filament Assembly and Disassembly Chih-Hao Lu, Wei-Hsuan Lan, and Hung-Wen Li
9. Generation of Fluorescent Versions of Saccharomyces cerevisiae RPA to Study the Conformational Dynamics of its ssDNA-binding Domains
Sahiti Kuppa, Nilisha Pokhrel, Elliot Corless, Sofia Origanti, and Edwin Antony
10. RPA-1 from Leishmania sp.: Recombinant Protein Expression and Purification, Molecular Modeling and Molecular Dynamics Simulations Protocols
Carlos A. H. Fernandes, Edna G. O. Morea, and Maria Isabel. N. Cano
11. Single-stranded DNA Curtains for Single-molecule Visualization of Rad51-ssDNA Filament Dynamics
Upasana Roy and Eric C. Greene
12. Following Trypanosoma cruzi RPA-DNA Interaction Using Fluorescent in Situ Hybridization Coupled with Immunofluorescence (FISH/IF)
Raphael S. Pavani and Maria Carolina Elias
13. Quantifying the affinity of Trypanosoma cruzi RPA-1 to the Single-stranded DNA Overhang of the Telomere using Surface Plasmon Resonance
Marcela de Oliveira Vitarelli and Maria Carolina Elias
14. Expression, Purification, and Solution-State NMR Analysis of the Two Human Single-stranded DNA Binding Proteins hSSB1 (NABP2/OBFC2B) and hSSB2 (NAPB1/OBFC2A)
Serene El-Kamand, Mar-Dean Du Plessis, Teegan Lawson, Liza Cubeddu, and Roland Gamsjaeger
15. Atomic Force Microscopy Reveals that the Drosophila Telomere-Capping Protein Verrocchio is a Single-stranded DNA Binding Protein
Alessandro Cicconi, Emanuela Micheli, Grazia Daniela Raffa, and Stefano Cacchione
16. Analysis of Mitochondrial SSB-DNA Complexes and their Effects on DNA Polymerase γ Activity by Electron Microscopy and Enzymatic Assays
Oya Bermek and Grzegorz L. Ciesielski
17. Optical Tweezers to Investigate the Structure and Energetics of Single-Stranded DNA-binding Protein-DNA Complexes
José A. Morin, Fernando Cerrón, Francisco J. Cao-García, and Borja Ibarra
18. Measurements of Real-time Replication Kinetics of DNA Polymerases on ssDNA Templates Coated with Single-stranded DNA-binding Proteins
Fernando Cerrón and Borja Ibarra
19. Selective Suppression of Endogenous Gene Expression Using RNAi in Drosophil
