1. The Ras Superfamily of Small GTPases: The Unlocked Secrets
Luca Goitre, Eliana Trapani, Lorenza Trabalzini, and Saverio Francesco Retta
2. A Novel Method for the Production of Fully-Modified K-Ras 4B
Tanmay S. Chavan, James O. Meyer, Luke Chisholm, Malgorzata Dobosz, and Vadim Gaponenko
3. Metabolic Labeling of Ras with Tritiated Palmitate to Monitor Palmitoylation and Depalmitoylation
Frederick D. Tsai, Joseph P. Wynne, Ian M. Ahearn, and Mark R. Philips
4. Ubiquitination of Rac1 by Inhibitors of Apoptosis (IAPs)
Tripat Kaur Oberoi-Khanuja and Krishnaraj Rajalingam
5. Ras GTPases are Both Regulators and Effectors of Redox Agents
Elisa Ferro, Luca Goitre, Eva Baldini, Saverio Francesco Retta, and Lorenza Trabalzini
6. Biophysical and Proteomic Characterization Strategies for Cysteine Modifications in Ras GTPases
G. Aaron Hobbs, Harsha P. Gunawardena, and Sharon L. Campbell
7. Use of the Yeast Two-Hybrid Technology to Isolate Molecular Interactions of Ras GTPases
Elisa Ferro, Eva Baldini, and Lorenza Trabalzini
8. Screening for MAPK Modulators Using an In-Cell Western Assay
Simon Schnaiter, Beatrix Fürst, Johannes Neu, Frigyes Wáczek, László Őrfi, György Kéri, Lukas A. Huber, and Winfried Wunderlich
9. Behavioral Methods for the Study of the Ras-ERK Pathway in Memory Formation and Consolidation: Passive Avoidance and Novel Object Recognition Tests
Raffaele d'Isa, Riccardo Brambilla, and Stefania Fasano
10. Functional Phosphoproteomics of Oncogenic KRAS Signaling
Putty-Reddy Sudhir and Jeou-Yuan Chen
11. Pull-Down Assay for Analysis of Integrin-Mediated Activation of Rap Proteins in Adherent Platelets
Gianni Francesco Guidetti and Mauro Torti
12. Combined Pulldown and Time-Lapse Microscopy Studies for Determining the Role of Rap1 in the Crosstalk between Integrins and Cadherins
Luca Goitre, Alessandro Morina, and Saverio Francesco Retta
13. Fluorescence Microscopy Study of Rap1 Subcellular Localization
Luca Goitre, Valentina Cutano, and Saverio Francesco Retta
14. An In Vitro System to Evaluate the Scaffold Function of the RalA Effector Protein RalBP1
David F. Kashatus
15. Analysis of the Rit Subfamily GTPase-Mediated Signaling and Neuronal Differentiation and Survival
Geng-Xian Shi and Catherine N. Kaminski
16. Immunofluorescence Methods in Studies of the GTPase RAN and Its Effectors in Interphase and in Mitotic Cells
Giulia Guarguaglini, Valeria de Turris, and Patrizia Lavia
17. High Resolution Scanning Electron Microscopy for the Imaging of Nuclear Pore Complexes and Ran-Mediated Transport
Lihi Shaulov, Boris Fichtman, and Amnon Harel
18. Effector Recruitment Method to Study Spatially Regulated Activation of Ras and Rho GTPases
Lauren P. Huff, Molly J. DeCristo, and Adrienne D. Cox
19. Real-Time Visualization and Quantification of Native Ras Activation in Single Living Cells
Christoph Biskup and Ignacio Rubio
20. Nanoclustering and Heterogeneous Membrane Diffusion of Ras Studied by FRAP and RICS Analysis
Camilo Guzmán, Maja Solman, and Daniel Abankwa
21. Analyzing the Roles of Rho GTPases in Cancer Cell Migration with a Live Cell Imaging 3D-Morphology-Based Assay
Audrey Colomba and Anne J. Ridley
22. Analysis of Rho-GTPase-Induced Localization of Nanoscale Adhesions Using Fluorescence Nanoscopy
Annica K.B. Gad, Daniel Rönnlund, Jerker Widengren, and Pontus Aspenström
23. Yeast as a Model for Ras Signaling
Renata Tisi, Fiorella Belotti, and Enzo Martegani
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