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Proteine im Phloem

Proteine im Phloem


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About the Book

Die vorliegende Studie beschäftigt sich mit Schutzmechanismen, die im Leitsystem des Phloems nach Applikation von mikrobiellen Elicitoren, so genannten Microbe-associated-molecular-patterns (MAMPs), zu finden sind. Konkret wurde ein potenzieller proteinvermittelter Siebelementverschluss der Versuchspflanzen Vicia faba und Cucurbita maxima nach flg22 (Flagellin) und glc8 (Chitin) Applikation untersucht. Im Phloem von Vicia faba, welche zur Familie der Fabaceae (Schmetterlingsblütler) zählen, existieren große spindelförmige Proteine, die bei einer Verletzung der Pflanze dispergieren. Diese Dispersion führt zu einem Verschluss der Siebelemente. Die phloemspezifischen Proteine in den Fabaceae heißen Forisome und beteiligen sich aktiv am Schutz der Pflanze (van Bel, A. J. E. et al. 2003). Das Ziel der Experimente an Vicia faba bestand darin, eine Reaktion der Forisome auf glc8 und flg22 zu überprüfen. Ebenfalls sollte ein Zusammenhang zwischen Lage und Position der Forisome im Siebelemente und der Reaktivität dokumentiert werden. In der Pflanze existieren verschiedene Arten von Abwehrreaktionen auf biotische Reize. Die systemische Route von Abwehrreaktionen verläuft vermutlich über das Phloem und wird vorwiegend über vorsorglich synthetisierte Substanzen, wie phloemspezifische Proteine (P-Proteine) organisiert. Daher liegt der Schwerpunkt dieser Arbeit in der Untersuchung der proteinvermittelten Verschlussmechanismen im Phloem. Im Fokus der Arbeit stehen die physiologischen Veränderungen der Forisome in Vicia faba nach MAMP-Applikation. Ziel dieser Methode war es, eine Reaktion der Forisome auf MAMPs zu generieren und somit einen Beweis für eine Abwehrantwort der Pflanze zu erhalten. Durch die Versuche sollte gezeigt werden, das Forisome ebenfalls eine Reaktion der Pattern-Triggerd-Immunity (PTI) sind. Des Weiteren sollten auch die Phloem-Proteine PP1 und PP2 von Cucurbita maxima auf eine MAMP-Reaktion überprüft werden, die eine Abwehrreaktion der Pflanze auf MAMP-App


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Product Details
  • ISBN-13: 9783955494650
  • Publisher: Bachelor + Master Publishing
  • Binding: Paperback
  • Language: German
  • Returnable: N
  • Spine Width: 4 mm
  • Weight: 190 gr
  • ISBN-10: 3955494659
  • Publisher Date: 11 Sep 2013
  • Height: 254 mm
  • No of Pages: 70
  • Series Title: German
  • Sub Title: Eine phytopathologische Untersuchung
  • Width: 178 mm


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