Preface...
Table of Contents...
Contributing Authors...
1. Diagnostic Imaging: Ultrasound
Stefan C. Kane, Su Lynn Khong, and Fabricio da Silva Costa
2. Biomarker Immunoassays in the Diagnosis of Pre-Eclampsia: Calculating the sFlt1: PlGF Ratio Using the Cobas e 411 Analyzer
Carin Black and Fabricio da Silva Costa
3. Assessing the Circulating Placental Specific Anti-Angiogenic Protein sFLT-1 e15a in Pre-Eclampsia
Kirsten Palmer
4. Role of Activin A in the Pathogenesis of Endothelial Cell Dysfunction in Pre-Eclampsia
Sebastian R. Hobson, Rebecca Lim, Joanne C. Mockler, Seshini Gurusinghe, and Euan M. Wallace
5. Genetic Approaches in Pre-Eclampsia
Hannah E. J. Yong, Padma Murthi, Shaun P. Brennecke, and Eric K. Moses
6. Epigenetics and Pre-Eclampsia: Programming of Future Outcomes
Alberto Borges Peixoto, Liliam Cristine Rolo, Luciano Marcondes Machado Nardozza, and Edward Araujo Júnior
7. Inflammatory and Immune System Markers
Kelly J. McKelvey, Gaayathri Ariyakumar, and Sharon A. McCracken
8. Methods to Enrich Exosomes from Conditioned Media and Biological Fluids
Shayna Sharma, Katherin Scholz-Romero, Gregory E. Rice, and Carlos Salomon
9. Isolation and Characterization of Extracellular Vesicles from Ex Vivo Cultured Human Placental Explants
Mancy Tong and Larry W. Chamley
10. Optimized Specific Isolation of Placenta-Derived Exosomes from Maternal Circulation
Andrew Lai, Omar Elfeky, Gregory E. Rice, and Carlos Salomon
11. Proteomics Method to Identification of Protein Profiles in Exosomes
Vyjayanthi Kinhal, Andrew Lai, Zarin Nuzhat, Ramkumar Menon, Gregory E. Rice, and Carlos Salomon
12. Harvesting and Characterization of Syncytial Nuclear Aggregates following Culture of First Trimester Human Placental Explants
Priyadarshini Pantham and Larry W. Chamley
13. Use of GATA3 and TWIST1 Immunofluorescence Staining to Assess In Vitro Syncytial Fusion Index
Severine A. Degrelle and Thierry Fournier
14. Ex Vivo Dual Perfusion of the Human Placenta: Disease Simulation, Therapeutic Pharmacokinetics and Analysis of their Off-Target Effects
Paul Brownbill, Neil Sebire, Erin V. McGillick, Stacey Ellery, and Padma Murthi
15. Immunohistological Techniques
Evangelina Capobianco and Nora Martinez
16. Using a Next Generation Sequencing Approach to Profile MicroRNAs from Human Origin
Dominic Guanzon, Juvita Delancy Iljas, Gregory E. Rice, and Carlos Salomon
17. Isolation and Purification of Villous Cytotrophoblast Cells from Term Human Placenta
Hélène Clabault, Laetitia Laurent, J. Thomas Sanderson, and Cathy Vaillancourt
18. Analyzing Trophoblast Function Using Cell-Based Assays
Katie L. Powell and Anthony W. Ashton
19. Isolation and Characterization of Mesenchymal Stem/Stromal Cells Derived from Human Third Trimester Placental Chorionic Villi and decidua basalis
Gina D. Kusuma, Mohamed H. Abumaree, Mark D. Pertile, and Bill Kalionis
20. An Electrical Impedance-Based Assay to Examine Functions of Various Placental Cell Types In Vitro
Tejasvy Chollangi, Hélène Clabault, Andrée-Anne Hudon Thibeault, Hannah E. J. Yong, Shagun Narula, Ellen Menkhorst, J. Thomas Sanderson, Cathy Vaillancourt, and Padma Murthi
21. In Vitro Induction of Hypoxia/Reoxygenation on Placental Cells: A Suitable Model for Understanding Placental Diseases
Lucas Sagrillo-Fagundes, Laetitia Laurent, and Cathy Vaillancourt
22. Measurement of Oxidative Stress-Mitochondrial Function Using the Seahorse System
Dilys TH Leung and Simon Chu
23. Co-Culture of H295R Adrenocortical Carcinoma and BeWo Choriocarcinoma Cells to Study Feto-Placental Interactions: Focus on Estrogen Biosynthesis
Andrée-Anne Hudon Thibeault, J. Thomas Sanderson, and Cathy Vaillancourt
24. Place
