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Polycomb Group Proteins

Polycomb Group Proteins


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About the Book

Mapping the Function of Polycomb Proteins.- Chromatin Immunoprecipitation.- Chromatin preparation and Chromatin Immuno-Precipitation from Drosophila embryos.- ChIP-seq data processing for PcG proteins and associated histone modifications.- Analysis of single locus replication timing in asynchronous cycling cells.- Non coding RNA interplay with the genome.- RIP: RNA Immunoprecipitation.- Capture Hybridization Analysis of DNA Targets.- Identification of RNA-protein interactions through in-vitro RNA pull-down assays.- Understanding RNA-chromatin interactions using Chromatin Isolation by RNA Purification (ChIRP).- Analysis RNA-seq and non coding RNA.- The dynamics of Polycomb complexes.- Fluorescence resonance energy transfer microscopy for measuring chromatin complex structure and dynamics.- Analysis of endogenous protein interactions of Polycomb-group of proteins in mouse embryonic stem cells.- Determination of Polycomb group of proteins compartmentalization through chromatin fractionation procedure.- An Automatic Segmentation Method Combining an Active Contour Model and a Classification Technique for Detecting Polycomb-group Proteins in High-Throughput Microscopy Images.- Polymer physics of the large-scale structure of chromatin.- Chromosome Conformation Capture in Drosophila.- Chromosome Conformation Capture in primary human cells.- Determination of high-resolution 3D chromatin organization using Circular Chromosome Conformation Capture (4C-seq).- Chromosome Conformation Capture on Chip (4C): Data Processing.- In vivo models to address the function of Polycomb Group Proteins.- A rapid TALEN assembly protocol.- Following the motion of Polycomb bodies in living Drosophila embryos.- Reprogramming of Somatic Cells towards Pluripotency by Cell Fusion.- Imaginal discs transplantation in Drosophila.- Isolation and culture of muscle stem cells.


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Product Details
  • ISBN-13: 9781493963782
  • Publisher: Humana Press
  • Publisher Imprint: Humana Press
  • Depth: 25
  • Language: English
  • Returnable: Y
  • Spine Width: 21 mm
  • Weight: 865 gr
  • ISBN-10: 1493963783
  • Publisher Date: 23 Sep 2016
  • Binding: Hardback
  • Height: 254 mm
  • No of Pages: 325
  • Series Title: Methods in Molecular Biology
  • Sub Title: Methods and Protocols
  • Width: 178 mm


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