1. Suppressor Screens in Arabidopsis
Xin Li and Yuelin Zhang
2. Quantitative Reverse Transcription-qPCR-Based Gene Expression Analysis in Plants
Heithem Ben Abdallah and Petra Bauer
3. DNA-Binding Factor Target Identification by Chromatin Immunoprecipitation (ChIP) in Plants
David Posé and Levi Yant
4. Identification of Genes Responsible for Natural Variation in Volatile Content Using Next Generation Sequencing Technology
Iraida Amaya, Jeremy Pillet, and Kevin M. Folta
5. Micro-Tom Tomato as an Alternative Plant Model System: Mutant Collection and Efficient Transformation
Masahito Shikata and Hiroshi Ezura
6. Culture of the Tomato Micro-Tom Cultivar in Greenhouse
Christophe Rothan, Daniel Just, Lucie Fernandez, Isabelle Atienza, Patricia Ballias, and Martine Lemaire-Chamley
7. Virus-Induced Gene Silencing as a Tool to Study Tomato Fruit Biochemistry
Elio Fantini and Giovanni Giuliano
8. A Simplified and Rapid Method for the Isolation and Transfection of Arabidopsis Leaf Mesophyll Protoplasts for Large-Scale Applications
Arnaldo L. Schapire and L. Maria Lois
9. Preparation of Epidermal Peels and Guard Cell Protoplasts for Cellular, Electrophysiological, and -Omics Assays of Guard Cell Function
Mengmeng Zhu, Byeong Wook Jeon, Sisi Geng, Yunqing Yu, Kelly Balmant, Sixue Chen, and Sarah M. Assmann
10. Application of Variable Angle Total Internal Reflection Fluorescence Microscopy to Investigate Protein Dynamics in Intact Plant Cells
Yinglang Wan, Yiqun Xue, Ruili Li, and Jinxing Lin
11. Immunoprecipitation of Plasma Membrane Receptor-Like Kinases for Identification of Phosphorylation Sites and Associated Proteins
Yasuhiro Kadota, Alberto P. Macho, and Cyril Zipfel
12. Yeast Three-Hybrid System for the Detection of Protein-Protein Interactions
Natsumi Maruta, Yuri Trusov, and Jose R. Botella
13. Cautions in Measuring In Vivo Interactions Using FRET and BiFC in Nicotiana benthamiana
Meral Tunc-Ozdemir, Yan Fu, and Alan M. Jones
14. Analysis of Protein-Lipid Interactions Using Purified C2 Domains
Jessica Pérez-Sancho, Arnaldo L. Schapire, Miguel A. Botella, and Abel Rosado
15. Assessing Kinase Activity in Plants with In-Gel Kinase Assays
Pengcheng Wang and Jian-Kang Zhu
16. Analyses of Plant UDP-Dependent Glycosyltransferases to Identify Their Volatile Substrates Using Recombinant Proteins
Yusuke Kamiyoshihara, Denise M. Tieman, and Harry J. Klee
17. Competitive Index: Mixed Infection-Based Virulence Assays for Genetic Analysis in Pseudomonas syringae-Plant Interactions
Alberto P. Macho, José S. Rufián, Javier Ruiz-Albert, and Carmen R. Beuzón
18. Simplified Assays for Evaluation of Resistance to Alternaria brassicicola and Turnip Mosaic Virus
Yuri Trusov, Ralf G. Dietzgen, Natsumi Maruta, and Jose R. Botella
19. Simultaneous Determination of Plant Hormones by GC-TOF-MS
José G. Vallarino and Sonia Osorio