1. Guide to the Book Plant Kinases
Nico Dissmeyer and Arp Schnittger
2. The Age of Protein Kinases
Nico Dissmeyer and Arp Schnittger
3. Expression and Purification of Active Protein Kinases from Wheat Germ Extracts
Boglárka Sonkoly, Viola Bardóczy, and Tamás Mészáros
4. Measurement of Plant Cyclin-Dependent Kinase Activity Using Immunoprecipitation-Coupled and Affinity Purification-Based Kinase Assays and the Baculovirus Expression System
Hirofumi Harashima and Masami Sekine
5. Mitogen-Activated Protein Kinase Activity and Reporter Gene Assays in Plants
Róbert Dóczi, Elizabeth Hatzimasoura, and László Bögre
6. Use of Phospho-site Substitutions to Analyze the Biological Relevance of Phosphorylation Events in Regulatory Networks
Nico Dissmeyer and Arp Schnittger
7. Bacterial Assay to Study Plant Sensor Histidine Kinases
Lukás Spíchal
8. Substrate Analysis of Arabidopsis PP2C-type Protein Phosphatase
Julija Umbrasaite, Alois Schweighofer and Irute Meskiene
9. Modulating and Monitoring MAPK Activity During Programmed Cell Death in Pollen
Shutian Li and Vernonica E. Franklin-Tong
10. Sensitizing Plant Protein Kinases to Specific Inhibition by ATP-Competitive Molecules
Dor Salomon, Chao Zhang, Kevan M. Shokat, and Guido Sessa
11. Fluorescence Fluctuation Analysis of Receptor Kinase Dimerization
Mark A. Hink, Sacco C. de Vries, and Antonie J.W.G. Visser
12. Quantifying Degradation Rates of Trans-Membrane Receptor Kinases
Niko Geldner
13. Fluorescence Correlation Spectroscopy and Fluorescence Recovery after Photobleaching to Study Receptor Kinase Mobility in planta
Mark A.C.J. Kwaaitaal, Marieke Schor, Mark A. Hink, Antonie J.W.G. Visser, and Sacco C. de Vries
14. Bimolecular-Fluorescence Complementation Assay to Monitor Kinase-Substrate Interactions in vivo
Stefan Pusch, Nico Dissmeyer, and Arp Schnittger
15. Chemical Genetic Analysis of Protein Kinase Function in Plants
Mike Böhmer, Michael Bölker, and Tina Romeis
16. Modified Metal Oxide Affinity Enrichment Combined with 2D-PAGE and Analysis of Phosphoproteomes
Thomas Colby, Horst Röhrig, Anne Harzen, and Jürgen Schmidt
17. Phosphoproteomics Using iTRAQ
Alexandra M.E. Jones and Thomas S. Nühse
