About the Book
1. Isolation of Peroxisomes from Rat Liver and Cultured Hepatoma Cells by Density Gradient Centrifugation
Andreas Manner and Markus Islinger
2. Isolation of Peroxisomes from Mouse Brain Using a Continuous Nycodenz Gradient: A Comparison to the Isolation of Liver and Kidney Peroxisomes
Miriam J. Schönenberger and Werner J. Kovacs
3. Determining the Topology of Peroxisomal Proteins Using Protease Protection Assays
Tânia Francisco, Ana F. Dias, Ana G. Pedrosa, Cláudia P. Grou, Tony A. Rodrigues, and Jorge E. Azevedo
4. Isolation of Native Soluble and Membrane-Bound Protein Complexes from Yeast Saccharomyces Cerevisiae
Tobias Hansen, Anna Chan, Thomas Schröter, Daniel Schwerter, Wolfgang Girzalsky, and Ralf Erdmann
5. Method for Measurement of Peroxisomal Very Long-Chain Fatty Acid Beta-Oxidation and de novo C26:0 Synthesis Activity in Living Cells Using Stable-Isotope Labelled Docosanoic Acid
Malu-Clair van de Beek, Inge M.E. Dijkstra, and Stephan Kemp
6. Analysis of Plasmalogen Synthesis in Cultured Cells
Masanori Honsho and Yukio Fujiki
7. Transfection of Primary Human Skin Fibroblasts for Peroxisomal Studies
Janet Koster and Hans R. Waterham
8. siRNA-Mediated Silencing of Peroxisomal Genes in Mammalian Cells
Tina A. Schrader and Michael Schrader
9. Dual Reporter Systems for the Analysis of Translational Readthrough in Mammals
Julia Hofhuis, Severin Dieterle, Rosemol George, Fabian Schueren, and Sven Thoms
10. Cytochemical Detection of Peroxisomes in Light and Electron Microscopy with 3,3'-diaminobenzidine
H. Dariush Fahimi
11. Cryo-Immuno Electron Microscopy of Peroxisomal Marker Proteins
Karina Mildner and Dagmar Zeuschner
12. Detection and Immunolabelling of Peroxisomal Proteins
Tina Schrader, Markus Islinger, and Michael Schrader
13. Labelling of Peroxisomes for Live Cell Imaging in the Filamentous Fungus Ustilago Maydis
Sofia C. Guimaraes, Sreedhar Kilaru, Michael Schrader, and Martin Schuster
14. Quantitative Monitoring of Subcellular Redox Dynamics in Living Mammalian Cells Using RoGFP2-based Probes
Celien Lismont, Paul A. Walton, and Marc Fransen
15. KillerRed as a Tool to Study the Cellular Responses to Peroxisome-Derived Oxidative Stress
Marc Fransen and Chantal Brees
16. Determination of Peroxisomal pH in Living Mammalian Cells Using pHRed
Luis F. Godinho, and Michael Schrader
17. In Cellulo Approaches to Study Peroxisomal Protein Import - Yeast Immunofluorescence Microscopy
Tobias Hansen, Wolfgang Girzalsky, and Ralf Erdmann
18. Blue Native Page: Applications to Study Peroxisome Biogenesis
Kanji Okumoto, Shigehiko Tamura, and Yukio Fujiki
19. In Vitro PMP Import Analysis Using Cell-Free Synthesized PMP and Isolated Peroxisomes
Yuqiong Liu, Masanori Honsho, and Yukio Fujiki
20. Peroxisomal Membrane and Matrix Protein Import Using a Semi-Intact Mammalian Cell System
Kanji Okumoto, Masanori Honsho, Yuqiong Liu, and Yukio Fujiki
21. The Use of Glycosylation Tags as Reporters for Protein Entry into the Endoplasmic Reticulum in Yeast and Mammalian Cells
Judith Buentzel, and Sven Thoms
22. Detection of Ubiquitinated Peroxisomal Proteins in Yeast
Natasha Danda and Chris Williams
23. Assessing Pexophagy in Mammalian Cells
Shun-ichi Yamashita and Yukio Fujiki
24. Experimental Systems to Study Yeast Pexophagy
Shun-ichi Yamashita, Masahide O
