Part I: Protocols for Establishment of Dental Cells Lines and Animal Models

1. Micro-dissection and Isolation of Mouse Dental Epithelial Cells of Continuously Growing Mouse Incisors

Hidemitsu Harada and Keishi Otsu

Wen'an Xu and Shuo Chen

3. Establishment of Stable Cell Lines from Primary Human Dental Pulp Stem Cells

Elizabeth Guirado, Youbin Zhang, and Anne George

4. Isolation of Dental Stem Cell Enriched Populations from Continuously Growing Mouse Incisors

Anamaria Balic

5. Application of Cell Lineage Tracing Combined with Immunofluorescence in the Study of Dentogenesis

Yan Jing, Chaoyuan Li, and Jian Q. Feng

6. Tissue Recombination and Kidney Capsule Transplantation Assays for the Study of Epithelial-mesenchymal Interactions

Lucia Jimenez-Rojo and Thimios Mitsiadis

7. Dental Mesenchymal Stem Cells: Dental Pulp Stem Cells, Periodontal Ligament Stem Cells, Apical Papilla Stem Cells, Primary Teeth Stem Cells: Isolation, Characterization and Expansion for Tissue Engineering

Mey Al-Habib and George T.-J. Huang

Part II: Protocols for Dental Stem Cells and Tissue Regeneration

8. Phenotypic Identification of Dental Pulp Mesenchymal Stem/Stromal Cells Subpopulations with Multiparametric Flow Cytometry

Maxime Ducret, Jean-Christophe Farges, Marielle Pasdeloup, Emeline Perrier-Groult, Andreas Mueller, Frédéric Mallein-Gerin, and Hugo Fabre

9. Dental Pulp Stem Cells: Isolation, Characterization, Expansion and Odontoblast Differentiation for Tissue Engineering

10. In vitro Analysis of Intramolecular Signaling Events in PDLSCs using Confocal and TIRF Microscopy

Annette Merkel and Anne George

11. A Mouse Model to Study Reparative Dentinogenesis

Babb RC, Chandrasekaran D, Zaugg LK, and Sharpe PT

12. Multiwalled Carbon Nanotubes for Dental Applications

Petros Kechagioglou;Eleftherios Andriotis; Petros Papagerakis; and Silvana Papagerakis

13. Peptide-mediated Biomimetic Regrowth of Human Enamel in situ

Kaushik Mukherjee, Qichao Ruan, and Janet Moradian-Oldak

Elizabeth E. Smith and Pamela C. Yelick

15. Whole-mount In Situ Hybridization of Mouse Embryos Using DIG-labeled RNA Probes

Jingyi Wu and Xiaofang Wang

Part III: Protocols for Studying Gene and Protein Expression

16. In Situ Hybridization on Mouse Paraffin Sections Using DIG-labeled RNA Probes

Jingyi Wu, Jian Q. Feng, and Xiaofang Wang

17. Methods for in situ Proteins Visualization in Dental Mineralized Tissues

D. Hotton, A. Berdal, and A. Bolanos

18. In Situ Hibridization in Mineralized Tissues: The Added Value of LNA Probes for RNA Detection

Lignon G, Hotton D, Berdal A, and Bolanos A

Xu Yang and Elia Beniash

20. Silver-albumin Tissue Staining Protocol to Visualize Odontogenesis in Whole Embryos

Julia C. Boughner and David M. L. Cooper

21. Isolation of SIBLING Proteins from Bone and Dentin Matrix

Jingyi Wu and Xiaofang Wang