Part I. Neutrophils: an Overview

1. The Role of Neutrophils in the Immune System: An Overview

Harry L. Malech, Frank R. DeLeo, and Mark T. Quinn

Part II. Neutrophil Isolation and Subcellular Fractionation

2. Isolation of Human Neutrophils

William M. Nauseef

3. Neutrophil Isolation from Non-Human Species

Daniel W. Siemsen, Natalia Malachowa, Igor A. Schepetkin, Adeline R. Whitney, Lilya N. Kirpotina, Benfang Lei, Frank R. DeLeo, and Mark T. Quinn

4. Collection of In vivo Transmigrated Neutrophils from Human Skin

Karin Christenson, Lena Björkman, Lisa Davidsson, Anna Karlsson, Per Follin, Claes Dahlgren, and Johan Bylund

5. Subcellular Fractionation of Human Neutrophils and Analysis of Subcellular Markers

Stine Novrup Clemmensen, Lene Udby, and Niels Borregaard

Part III. Biochemistry, Biology and Signal Transduction of Neutrophils

6. Rho Family and Rap GTPase Activation Assays

Richard T. Jennings and Ulla G. Knaus

7. Measurement of Phospholipid Metabolism in Intact Neutrophils

Susan Sergeant and Linda C. McPhail

8. Optical Methods for the Measurement and Manipulation of Cytosolic Free Calcium Signals in Neutrophils

Maurice B. Hallett, Maha Al-Jumaa, and Sharon Dewitt

9. Analysis of Electrophysiological Properties and Responses of Neutrophils

Deri Morgan and Thomas E. DeCoursey

10. Analysis of Neutrophil Apoptosis

David A. Dorward, Adriano G. Rossi, Ian Dransfield, and Christopher D. Lucas

11. Microinjection Methods for Neutrophils

Iraj Laffafian, Kimberly J. Lewis, K. Benjamin Masterman, and Maurice B. Hallett

12. Generation of Functionally Mature Neutrophils from Induced Pluripotent Stem Cells

Colin L. Sweeney, Randall K. Merling, Uimook Choi, Debra Long Priel, Douglas B. Kuhns, Hongmei Wang, and Harry L. Malech

Part IV. Neutrophil Adhesion and Chemotaxis

13. Neutrophil Migration Through Extracellular Matrix

Richard T. Jennings and Ulla G. Knaus

14. Spinning Disk Confocal Imaging of Neutrophil Migration in Zebrafish

Pui-ying Lam, Robert S. Fischer, William D. Shin, Clare M. Waterman, and Anna Huttenlocher

15. Detection of Bi-Directional Signalilng During Integrin Activation and In Neutrophil Adhesion

Stuart M. Altman, Neha Dixit, and Scott I. Simon

Part V. Neutrophil Phagocytosis and Bactericidal Activity

16. Immunofluorescence and Confocal Microscopy of Neutrophils

Lee-Ann H. Allen

17. Expression of Genetically-Encoded Fluorescent Probes to Monitor Phospholipid Dynamics in Live Neutrophils

Benjamin E. Steinberg, Marco A.O. Magalhaes, and Sergio Grinstein

18. Quantitative Assessment of Neutrophil Phagocytosis Using Flow Cytometry

Pontus Nordenfelt

19. Analysis of Neutrophil Bactericidal Activity

Heather A. Parker, Nicholas J. Magon, Jessie N. Green, Mark B. Hampton, and Christine C. Winterbourn

20. Induction and Quantification of Neutrophil Extracellular Traps

Alejandro Sanchez Gonzalez, Bart W. Bardoel, Christopher J. Harbort, and Arturo Zychlinsky

Part VI. NADPH Oxidase and Production of Reactive Oxygen Species

21. Measurement of Respiratory Burst Products, Released or Retained, During Activation of Professional Phagocytes

Johan Bylund, Halla Björnsdottir, Martina Sundqvist, Anna Karlsson, and Claes Dahlgren

22. Cell-free NADPH Oxidase Assays: "In vitro Veritas"

Edgar Pick

23. Assessment of Priming of the Human Neutrophil Respiratory Burst

Margarita Hurtado-Nedelec, Karama Makni-Maalej, Marie-Anne Gougerot-Pocidalo, Pham