1. Targeted Deletion of Hsf1, 2, and 4 Genes in Mice
Xiongjie Jin, Binnur Eroglu, Demetrius Moskophidis, and Nahid F. Mivechi
2. The Role Of Heat Shock Factors in Stress-Induced Transcription
Yue Zhang, Shiuh-Dih Chou, Ayesha Murshid, Thomas L. Prince, Sheila Schreiner, Mary Ann Stevenson, and Stuart K. Calderwood
3. Hsp90 and Client Protein Maturation
Natalie Wayne, Parul Mishra, and Daniel N. Bolon
4. The Role of p23, Hop, Immunophilins, and Other Co-Chaperones in Regulating Hsp90 Function
Marc B. Cox and Jill L. Johnson
5. Detecting Hsp90 Phosphorylation
Mehdi Mollapour and Len Neckers
6. Role of Molecular Chaperones in Biogenesis of the Protein Kinome
Atin K. Mandal, Maria A. Theodoraki, Nadinath B. Nillegoda, and Avrom J. Caplan
7. Nucleotide Exchange Factors for Hsp70 Chaperones
Heike Rampelt, Matthias P. Mayer, and Bernd Bukau
8. Reconstitution of CHIP E3 Ubiquitin Ligase Activity
Hong Yu Ren, Cam Patterson, Douglas M. Cyr, and Meredith F.N. Rosser
9. Structure-Functions of HspB1 (Hsp27)
André-Patrick Arrigo
10. Combined Lentiviral and RNAi Technologies for the Delivery and Permanently Silencing of the hsp25 Gene
Punit Kaur, Ganachari M. Nagaraja, Khalid Bin Abdulrahman, and Alexzander Asea
11. Quantification of HSP27 and HSP70 Molecular Chaperone Activities
Jessica Gobbo, Caroline Gaucher-Di-Stasio, Stéphanie Weidmann, Jean Guzzo, and Carmen Garrido
12. Measuring Hsp72 (HSPA1A) by Indirect Sandwich ELISA
H. Elyse Ireland and John H.H. Williams
13. Analysis of Heat Shock Protein Localization Using Flow Cytometry
Nina C. Dempsey, Francesca Leoni, Christine Hoyle, and John. H.H. Williams
14. Quantitation of Heat Shock Proteins in Clinical Samples Using Mass Spectrometry
Punit Kaur, Khalid Bin Abdulrahman, and Alexzander Asea
15. Bioinformatic Approach to Identify Chaperone Pathway Relationship from Large-Scale Interaction Networks
Yunchen Gong, Zhaolei Zhang, and Walid A. Houry
16. Hsp70: Anti-Apoptotic and Tumorigenic Protein
Anne-Laure Rérole, Gaëtan Jego, and Carmen Garrido
17. Determination of Cell Survival or Death
Alexander E. Kabakov, Vladimir A. Kudryavtsev, and Vladimir L. Gabai
18. Immunohistochemistry of Human Hsp60 in Health and Disease: From Autoimmunity to Cancer
Francesco Cappello, Everly Conway de Macario, Giovanni Zummo, and Alberto J.L. Macario
19. Preparation of a Heat Shock Proteins 70-Based Vaccine from DC-Tumor Fusion Cells
Desheng Weng, Stuart K. Calderwood, and Jianlin Gong
20. Isolation of Heat Shock Protein Complexes
Fernando D. Cuello-Carrión, Mariel A. Fanelli, Niubys Cayado-Gutiérrez, Gisela Castro, and Daniel R. Ciocca
21. Enhancing Antigen Cross-Presentation and T-Cell Priming by Complexing Protein Antigen to Recombinant Large Heat Shock Protein
Xiang-Yang Wang, Huanfa Yi, Xiaofei Yu, Damin Zuo, and John R. Subjeck
22. Investigating Receptors for Extracellular Heat Shock Proteins
Ayesha Murshid, Jimmy Theriault, Jianlin Gong, and Stuart K. Calderwood
23. Analysis of Cellular Migration Using a Two-Chamber Methodology
