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Microrna Protocols

Microrna Protocols


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About the Book

The MicroRNA.- Analysis of MicroRNA Length Variety Generated by Recombinant Human Dicer.- A User-Friendly Computational Workflow for the Analysis of miRNA Deep Sequencing Data.- The RNA Gene Information: Retroelement-MicroRNA Entangling as the RNA Quantum Code.- Setting Up an Intronic miRNA Database.- Identify Intronic MicroRNA with Bioinformatics.- Experimental miRNA Target Validation.- Enrichment Analysis of miRNA Targets.- MicroRNA Expression Profiling During Neural Differentiation of Mouse Embryonic Carcinoma P19 Cells.- Evaluating the MicroRNA Targeting Sites by Luciferase Reporter Gene Assay.- Expression Pattern Analysis of MicroRNAs in Caenorhabditis elegans.- Use of Viral Systems to Study miRNA-Mediated Regulation of Gene Expression in Human Cells.- Cloning, Expression, and Functional Analysis of Genomic miRNA Using Retroviral System in Cancer Cells.- Preparing Synaptoneurosomes from Adult Mouse Forebrain.- Isolation of Total RNA and Detection Procedures for miRNA Present in Bovine Cultured Adipocytes and Adipose Tissues.- miRNA and shRNA Expression Vectors Based on mRNA and miRNA Processing.- Gene Silencing In Vitro and In Vivo Using Intronic MicroRNAs.- Vector-Free Methods for Manipulating miRNA Activity In Vitro and In Vivo.- MicroRNA Expression Profiling of Human Induced Pluripotent and Embryonic Stem Cells.- MicroRNA Expression During Neuronal Differentiation of Human Teratocarcinoma NTera2D1 and Mouse Embryonic Carcinoma P19 Cells.- Isolation and Identification of Gene-Specific MicroRNAs.- Transgene-Like Animal Models Using Intronic MicroRNAs.- Mechanism and Method for Generating Tumor-Free iPS Cells Using Intronic MicroRNA miR302 Induction.- Salivary MicroRNAs and Oral Cancer Detection.- Application of Intronic MicroRNA Agents in Cosmetics.- MicroRNAs in Skin and Wound Healing.- MiRNA Targets of Prostate Cancer.


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Product Details
  • ISBN-13: 9781627030823
  • Publisher: Humana Press
  • Publisher Imprint: Humana Press
  • Depth: 32
  • Height: 257 mm
  • No of Pages: 375
  • Series Title: Methods in Molecular Biology
  • Weight: 911 gr
  • ISBN-10: 1627030824
  • Publisher Date: 26 Sep 2012
  • Binding: Hardback
  • Edition: 2nd ed. 2013
  • Language: English
  • Returnable: Y
  • Spine Width: 28 mm
  • Width: 178 mm


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