1. Introduction to Glycosylation and Mass Spectrometry

Steven M. Patrie, Michael J. Roth and Jennifer J. Kohler

Part I - Enrichment and Isolation Methods

2. Tandem Lectin Weak Affinity Chromatography (LWAC) for Glycoprotein Enrichment

Zhi Yuan Ma, Yuliya Skorobogatko, and Keith Vosseller

3. CSC Technology - Selective Labeling of Glycoproteins by Mild Oxidation to Phenotype Cells

Andreas Hofmann, Damaris Bausch-Fluck, and Bernd Wollscheid

4. Use of Boronic Acid Nanoparticles in Glycoprotein Enrichment

Yawei Xu, Lijuan Zhang and Haojie Lu

5. Incorporation of Unnatural Sugars for the Identification of Glycoproteins

Balyn W. Zaro, Howard C. Hang, Matthew R. Pratt

6. Characterization of Membrane-associated Glycoproteins using Lectin Affinity Chromatography and Mass Spectrometry

Yashu Liu, Jintang He and David M. Lubman

7. Sialic Acid Capture-and-Release and LC-MSⁿ Analysis of Glycopeptides

Jonas Nilsson and Göran Larson

Part II - Sample Preparation

8. In-Solution Digestion of Glycoproteins for Glycopeptide-Based Mass Analysis

9. Nano-HPLC-MS of Glycopeptides Obtained After Non-specific Proteolysis

Gerhild Zauner, Carolien A. M. Koeleman, André M. Deelder and Manfred Wuhrer

Part III - Separation Methods

10. Glycopeptide Enrichment for MALDI-TOF Mass Spectrometry Analysis by Hydrophilic Interaction Liquid Chromatography Solid Phase Extraction (HILIC SPE)

Pia Hønnerup Jensen, Simon Mysling, Peter Højrup and Ole Nørregaard Jensen

11. Separation and Identification of Glycoforms by Capillary Electrophoresis with Electrospray Ionization Mass Spectrometric Detection

Alina D. Zamfir , Corina Flangea, Alina Serb³, Ana-Maria Zagrean⁴, Andreas M. Rizzi⁵ and Eugen Sisu

12. Structural Separations by Ion Mobility-MS for Glycomics and Glycoproteomics

Larissa S. Fenn and John A. McLean

Part IV - Quantitation Methods

13. Quantitative Analysis of Glycoprotein Glycans

Ron Orlando

14. Stable Isotope Labeling of N-glycosylated Peptides by Enzymatic Deglycosylation for Mass Spectrometry-based Glycoproteomics

Hiroyuki Kaji and Toshiaki Isobe

Peng Zhao, Stephanie H. Stalnaker, Lance Wells

16. Glycan Profiling: Label-free Analysis of Glycoproteins

Yoshinao Wada

Part V - Computational Tools

17. Introduction to Informatics in Glycoprotein Analysis

Kiyoko F. Aoki-Kinoshita

18. Software Tools for Glycan Profiling

Chuan-Yih Yu, Anoop Mayampurath, Haixu Tang

Part VI - Case Studies in Mass Spectrometry of Glycoproteins

19. Quantitative Characterization of Glycoproteins in Neurodegenerative Disorders using iTRAQ

Min Shi, Hyejin Hwang and Jing Zhang

Lei Zhou and Roger W. Beuerman

21. Elucidation of N-glycosites Within Human Plasma Glycoproteins for Cancer Biomarker Discovery

Penelope Drake, Birgit Schilling, Brad Gibson and Susan Fisher

22. Characterizing the Glycosylation State of Therapeutic Recombinant Glycoproteins

Nicole Samuels, David Kates, Jun Liu, Joanne Severs

11. Separation and Identification of Glycoforms by Capillary Electrophoresis with Electrospray Ionization Mass Spectrometric Detection

Alina D. Zamfir , Corina Flangea, Alina Serb³, Ana-Maria Zagrean⁴, Andreas M. Rizzi