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Home > Science & Mathematics > Biology, life sciences > Microbiology (non-medical) > lQ interaction with E. coli RNA polymerase for antitermination Regions of E. coli RNA polymerase required for lQ-mediated antitermination in binding and function.
lQ interaction with E. coli RNA polymerase for antitermination Regions of E. coli RNA polymerase required for lQ-mediated antitermination in binding and function.

lQ interaction with E. coli RNA polymerase for antitermination Regions of E. coli RNA polymerase required for lQ-mediated antitermination in binding and function.


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About the Book

Phage λQ protein is a transcriptional antiterminator that is required for late gene expression from the pR' promoter when λ prophage decides to follow the lytic pathway. λQ modifies Escherichia coli RNA polymerase (RNAP) at the early elongation stage. Once engaged, λQ maintains itself as a subunit of the elongation complex throughout the elongation. Studies show that λQ confers RNAP resistance to both intrinsic and ρ-mediated terminators and less pausing. However, little is known about the interaction between λQ and RNAP. In this study, the largest subunits (β and β') of RNAP were examined to map the λQ binding site. A stronger binding was observed with β than β', and the binding site was narrowed to β501-832. This region was further defined as small as 82 amino acids (β600-681) that interacted with λQ. In addition, 12 mutant RNAPs were isolated for a reduced λQ antitermination within the potential λQ binding regions, specifically near the main channel. These results propose that λQ modifies the active center of RNAP for antitermination.


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Product Details
  • ISBN-13: 9783639165814
  • Publisher: VDM Verlag
  • Binding: Paperback
  • Language: English
  • Returnable: N
  • Weight: 317 gr
  • ISBN-10: 3639165810
  • Publisher Date: 07 Jun 2009
  • Height: 229 mm
  • No of Pages: 176
  • Spine Width: 10 mm
  • Width: 152 mm


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lQ interaction with E. coli RNA polymerase for antitermination Regions of E. coli RNA polymerase required for lQ-mediated antitermination in binding and function.
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lQ interaction with E. coli RNA polymerase for antitermination Regions of E. coli RNA polymerase required for lQ-mediated antitermination in binding and function.
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