1. 4D Confocal Imaging of Yeast Organelles
Kasey J. Day, Effrosyni Papanikou, and Benjamin S. Glick
2. Imaging the Polarized Sorting of Proteins from the Golgi Complex in Live Neurons
Ginny G. Farías, Dylan J. Britt, and Juan S. Bonifacino
3. Imaging Golgi Outposts in Fixed and Living Neurons
Mariano Bisbal, Gonzalo Quassollo, and Alfredo Caceres
4. Analysis of Arf1 GTPase-Dependent Membrane Binding and Remodeling Using the Exomer Secretory Vesicle Cargo Adaptor
Jon E. Paczkowski and J. Christopher Fromme
5. STEM Tomography Imaging of Hypertrophied Golgi Stacks in Mucilage-Secreting Cells
Byung-Ho Kang
6. Reconstitution of COPI Vesicle and Tubule Formation
Seung-Yeol Park, Jia-Shu Yang, and Victor W. Hsu
7. Reconstitution of Phospholipase A2-Dependent Golgi Membrane Tubules Edward B. Cluett, Paul de Figueiredo, Marie E. Bechler, Kevin D. Thorsen, and William J. Brown
8. Proteomic Characterization of Golgi Membranes Enriched from Arabidopsis Suspension Cell Cultures
Sara Fasmer Hansen, Berit Ebert, Carsten Rautengarten, and Joshua L. Heazlewood
9. High Content Analysis of the Golgi Complex by Correlative Screening Microscopy
Manuel Gunkel, Holger Erfle, and Vytaute Starkuviene
10. Activity Detection of GalNAc Transferases by Protein-Based Fluorescence Sensors In Vivo
Lina Song, Collin Bachert, and Adam D. Linstedt
11. In Situ Proximity Ligation Assay (PLA) Analysis of Protein Complexes Formed Between Golgi-Resident, Glycosylation-Related Transporters and Transferases in Adherent Mammalian Cell Cultures
Dorota Maszczak-Seneczko, Paulina Sosicka, Teresa Olczak, and Mariusz Olczak
12. Creating Knock-Outs of Conserved Oligomeric Golgi Complex Subunits Using CRISPR-Mediated Gene Editing Paired with a Selection Strategy Based on Glycosylation Defects Associated with Impaired COG Complex Function
Jessica Bailey Blackburn and Vladimir V. Lupashin
13. Reversible Controlled Aggregation of Golgi Resident Enzymes to Assess Their Transport/Dynamics Along the Secretory Pathway
Riccardo Rizzo and Alberto Luini
14. Assays to Study the Fragmentation of the Golgi Complex During the G2/M Transition of the Cell Cycle
Inmaculada Ayala and Antonino Colanzi
15. The Role of Lysophospholipid Acyltransferases in the Golgi Complex
John A. Schmidt
16. Methods to Purify and Assay Secretory Pathway Kinases
Vincent S. Tagliabracci, Jianzhong Wen, and Junyu Xiao
