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The Golgi Complex

The Golgi Complex


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About the Book

1. 4D Confocal Imaging of Yeast Organelles

Kasey J. Day, Effrosyni Papanikou, and Benjamin S. Glick

2. Imaging the Polarized Sorting of Proteins from the Golgi Complex in Live Neurons

Ginny G. Farías, Dylan J. Britt, and Juan S. Bonifacino

3. Imaging Golgi Outposts in Fixed and Living Neurons

Mariano Bisbal, Gonzalo Quassollo, and Alfredo Caceres

4. Analysis of Arf1 GTPase-Dependent Membrane Binding and Remodeling Using the Exomer Secretory Vesicle Cargo Adaptor

Jon E. Paczkowski and J. Christopher Fromme

5. STEM Tomography Imaging of Hypertrophied Golgi Stacks in Mucilage-Secreting Cells

Byung-Ho Kang

6. Reconstitution of COPI Vesicle and Tubule Formation

Seung-Yeol Park, Jia-Shu Yang, and Victor W. Hsu

7. Reconstitution of Phospholipase A2-Dependent Golgi Membrane Tubules

Edward B. Cluett, Paul de Figueiredo, Marie E. Bechler, Kevin D. Thorsen, and William J. Brown

8. Proteomic Characterization of Golgi Membranes Enriched from Arabidopsis Suspension Cell Cultures

Sara Fasmer Hansen, Berit Ebert, Carsten Rautengarten, and Joshua L. Heazlewood

9. High Content Analysis of the Golgi Complex by Correlative Screening Microscopy

Manuel Gunkel, Holger Erfle, and Vytaute Starkuviene

10. Activity Detection of GalNAc Transferases by Protein-Based Fluorescence Sensors In Vivo

Lina Song, Collin Bachert, and Adam D. Linstedt

11. In Situ Proximity Ligation Assay (PLA) Analysis of Protein Complexes Formed Between Golgi-Resident, Glycosylation-Related Transporters and Transferases in Adherent Mammalian Cell Cultures

Dorota Maszczak-Seneczko, Paulina Sosicka, Teresa Olczak, and Mariusz Olczak

12. Creating Knock-Outs of Conserved Oligomeric Golgi Complex Subunits Using CRISPR-Mediated Gene Editing Paired with a Selection Strategy Based on Glycosylation Defects Associated with Impaired COG Complex Function

Jessica Bailey Blackburn and Vladimir V. Lupashin

13. Reversible Controlled Aggregation of Golgi Resident Enzymes to Assess Their Transport/Dynamics Along the Secretory Pathway

Riccardo Rizzo and Alberto Luini

14. Assays to Study the Fragmentation of the Golgi Complex During the G2/M Transition of the Cell Cycle

Inmaculada Ayala and Antonino Colanzi

15. The Role of Lysophospholipid Acyltransferases in the Golgi Complex

John A. Schmidt

16. Methods to Purify and Assay Secretory Pathway Kinases

Vincent S. Tagliabracci, Jianzhong Wen, and Junyu Xiao



Product Details
  • ISBN-13: 9781493964611
  • Publisher: Humana Press
  • Publisher Imprint: Humana Press
  • Edition: 1st ed. 2016
  • Language: English
  • Returnable: Y
  • Spine Width: 18 mm
  • Weight: 671 gr
  • ISBN-10: 1493964615
  • Publisher Date: 02 Nov 2016
  • Binding: Hardback
  • Height: 265 mm
  • No of Pages: 219
  • Series Title: Methods in Molecular Biology
  • Sub Title: Methods and Protocols
  • Width: 183 mm


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