I. Endogenous DNA: Cell Fusion
1. Anastomosis and Heterokaryon Formation
Martin Weichert and André Fleißner
2. Induction of the Sexual Cycle in Filamentous Ascomycetes
Jos Houbraken and Paul S. Dyer
3. What Have We Learned by Doing Transformations in Neurospora tetrasperma?
Durgadas P. Kasbekar
II. Endogenous DNA: Repetitive Elements
4. Repeat-induced Point Mutation: A Fungal-specific, Endogenous Mutagenesis Process
James K. Hane, Angela H. Williams, Adam P. Taranto, Peter S. Solomon and Richard P. Oliver
5. Calculating RIP Mutation in Fungal Genomes Using RIPCAL
James K. Hane
6. Fungal Transposable Elements Linda Paun and Frank Kempken
7. In vivo Targeted Mutagenesis in Yeast using TaGTEAM
Shawn Finney-Manchester and Narendra Maheshri
III. Endogenous DNA: RNAi
8. RNA Silencing in Filamentous Fungi: from Basic to Applications
Nguyen Bao Quoc and Hitoshi Nakayashiki
9. RNAi-mediated Gene Silencing in the Beta-lactam Producers Fungi Penicillium chrysogenum and Acremonium chrysogenum
Carlos García-Estrada and Ricardo V. Ullán
10. Controlling Fungal Gene Expression Using the Doxycycline-Dependent Tet-ON System in Aspergillus fumigatus
Michaela Dümig and Sven Krappmann
IV. Tools & Applications: Selection Markers and Vectors
11. Expanding the Repertoire of Selectable Markers for Aspergillus Transformation
Khyati Dave, V. Lakshmi Prabha, Manmeet Ahuja, Kashyap Dave, S. Tejaswini and Narayan S. Punekar
12. Arginase (agaA) as a Fungal Transformation Marker
Kashyap Dave, Manmeet Ahuja, Jayashri, T.N., Rekha Bisht Sirola, Khyati Dave and Narayan S. Punekar
13. Transformation of Ascomycetous Fungi using Autonomously Replicating Vectors
Takeo Shimizu and Satoko Kanematsu
14. A Recyclable and Bidirectionally Selectable Marker System for Transformation of Trichoderma
Thiago M. Mello-de-Sousa, Robert L. Mach and Astrid R. Mach-Aigner
15. Split Marker-Mediated Transformation and Targeted Gene Disruption in Filamentous Fungi
Kuang-Ren Chung and Miin-Huey Lee
V. Tools & Applications: High Throughput Experimentation
16. Integrated Automation for Continuous High-Throughput Synthetic Chromosome Assembly and Transformation to Identify Improved Yeast Strains for Industrial Production of Biofuels and Bio-based Chemicals
Stephen R. Hughes and Steven B. Riedmuller
17. Imaging Flow Cytometry and High-throughput Microscopy for Automated Macroscopic Morphological Analysis of Filamentous Fungi
Aydin Golabgir, Daniela Ehgartner, Lukas Neutsch, Andreas E. Posch, Peter Sagmeister and Christoph Herwig
18. Yeast Cell Electroporation in Droplet-Based Microfluidic Chip
Qiuxian Cai and Chunxiong Luo
19. Identification of T-DNA Integration Sites: TAIL-PCR and Sequence Analysis
Jaehyuk Choi, Junhyun Jeon and Yong-Hwan Lee
VI. Tools & Applications: Comprehensive Approaches in Selected Fungi
20. Genetic and Genomic Manipulation in Aspergillus niger
Adrian Tsang, Annie Bellemare, Corinne Darmond and Janny Bakhuis
21. Genetic Manipulation of Meyerozyma guilliermondii
Nicolas Papon, Yuriy R. Boretsky, Vincent Courdavault, Marc Clastre and Andriy A. Sibirny
About the Author: Marco A. van den Berg, PhD
Principal Scientist Applied Biochemistry and Screening
DSM Biotechnology Center
Delft, The Netherlands
Karunakaran Maruthachalam, PhD
Current: Research Scientist
Global Marker Technology Lab, DuPont-Pioneer
DuPont Knowledge Center
Andhra Pradesh, India
Previous: Molecular Biologist
California Seed and Plant Labs
Pleasant Grove, CA, USA
