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Food Allergens

Food Allergens


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About the Book

Preface...
Table of Contents...
Contributing Authors...

1: Overview of the Commonly used Methods for Food Allergens
Jing Lin and Marcos J.C. Alcocer

Part I Food Allergen Purification and Production

2: Allergen Extraction and Purification from Natural Products: Main Chromatographic Techniques
Barbara Cases, Carlos Pastor-Vargas, and Marina Perez-Gordo

3: Recombinant Allergen Production in E. Coli
Changqi Liu, LeAnna N. Willison, and Shridhar K. Sathe

4: Recombinant Allergens Production in Yeast
Maria Neophytou and Marcos J.C. Alcocer

Part II Food Allergen Discovery, Detection, and Quantification

5: 2D-Electrophoresis and Immunoblotting in Food Allergy
Galina Grishina, Luda Bardina, and Alexander Grishin

6: Two Dimensional Electrophoresis and Identification by Mass Spectrometry (MS)
Fernando de la Cyesta, Gloria Alvarez-Llamas, and Maria G. Barderas

7: Enzyme-Linked Immunosorbent Assay (ELISA)
George N. Konstantinou

8: Detection of Food Allergens by Taqman Real-Time PCR Methodology
Aina García, Raquel Madrid, Teresa García, Rosario Martín, and Isabel González

9: Detection of Food Allergens by Phage Displayed Produced Antibodies
Raquel Madrid, Silvia de la Cruz, Aina García, Rosario Martín, Isabel González, and Teresa García

10: Protein Microarray-Based IgE-Immunoassay for Allergy Diagnosis
Nuzul N. Jambari, XiaoWei Wang, and Marcos J.C. Alcocer

11: Basophil Degranulation Assay
Madhan Masilamani, Mohanapriya Kamalakannan, and Hugh A. Sampson

12: Use of Humanized RS-ATL8 Reporter System for Detection of Allergen-Specific IgE Sensitization in Human Food Allergy
Eman Ali Ali, Ryosuke Nakamura, and Franco H. Falcone

Part III Allergenic Epitope Mapping

13: Assessment of IgE Reactivity of β-Casein by Western Blotting after Digestion with Simulated Gastric Fluid
Sara Benedé, Rosina López-Fandiño, and Elena Molina

14: IgE Epitope Mapping using Peptide Microarray Immunoassay
Jing Lin and Hugh A. Sampson

15: T Cell Proliferation Assay: Determination of Immunodominant T Cell Epitopes of Food Allergens
Madhan Masilamani, Mariona Pascal, and Hugh A. Sampson

16: Tetramer Guided Epitope Mapping: A Rapid Approach to Identify HLA-Restricted T-Cell Epitopes from Composite Allergens
Luis Diego Archila and William W. Kwok

17: T-Cell Epitope Prediction
George N. Konstantinou

18: An Overview of Bioinformatics Tools and Resources in Allergy
Zhiyan Fu and Jing Lin

Part IV Methods Currently being Developed and Future Development

19: The Use of a Semi-Automated System to Measure Mouse Natural Killer T (NKT) Cell Activation by Lipid Loaded Dendritic Cells
Ashfaq Ghumra and Marcos J.C. Alcocer

20: Recent Advances in the Detection of Allergens in Foods
Silvia de la Crus, Inés López-Calleja, Rosario Martín, Isabel González, Marcos J.C. Alcocer, and Teresa García


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Product Details
  • ISBN-13: 9781493983407
  • Publisher: Humana Press
  • Publisher Imprint: Humana Press
  • Height: 254 mm
  • No of Pages: 299
  • Series Title: Methods in Molecular Biology
  • Sub Title: Methods and Protocols
  • Width: 178 mm
  • ISBN-10: 1493983407
  • Publisher Date: 07 Aug 2018
  • Binding: Paperback
  • Language: English
  • Returnable: N
  • Spine Width: 17 mm
  • Weight: 594 gr


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