Part I: Native Tissue
1. Immunohistochemical Characterization of Brain Neural and Vascular Basement Membranes
Zhen Huang
2. The Use of Synaptic Basal Lamina and Its Components to Identify Sites of Recent Morphological Alterations at Mammalian Neuromuscular Junctions
Young il Lee and Yue Li
3. Perineuronal Nets: A Special Structure in the Central Nervous System Extracellular Matrix
Jessica C.F. Kwok, Simona Foscarin, and James W. Fawcett
Part II: In Vitro Models
4. Isolation of Perinatal Mouse Cerebral Cortical Astrocytes
Rolicia F. Martin and Elizabeth M. Powell
5. Techniques for Examining the Effect of Substratum Bound Proteoglycans on Neurite Outgrowth In Vitro
Justin A. Beller, Thomas M. Hering, and Diane M. Snow
6. High-Throughput Quantitative Assay for Analyzing Neurite Outgrowth on a Uniform Substratum: The Cell-Substratum Assay
Justin A. Beller, Thomas M. Hering, and Diane M. Snow
7. Extracellular Matrix Components as a Substrate for Outgrowing Motoneurons
Rebecca Conrad, Alice Klausmeyer, Teresa Tsai, Andreas Faissner, and Stefan Wiese
8. Replicating the In Vivo Environment: Organotypic and Submerged Three-Dimensional Culture Methods
Céline Plachez and Elizabeth M. Powell
9. Culturing Neurons, Glia, and Progenitor Cells in Three-Dimensional Hydrogels
Swarnalatha Balasubramanian, Elizabeth M. Powell, and Jennie B. Leach
10. Investigating Cell-ECM Interactions and ECM Synthesis in Three-Dimensional Hydrogels
Swarnalatha Balasubramanian, Elizabeth M. Powell, and Jennie B. Leach
Part III: Injury, Degeneration, and Regeneration
11. Characterization and Analysis of Extracellular Matrix in Malignant Brain Tumors and Their Cellular Derivatives
Maksim Sinyuk, Justin D. Lathia, and Mariano S. Viapiano
12. Chondroitinase Gene Therapy for Spinal Cord Injury
Jianli Hu, Gabrielle M. Curinga, and George M. Smith
13. Modulation of Neural Stem Cell Expressed Extracellular Matrix (ECM) by Targeting Glycosyltransferases
Eva Hennen and Andreas Faissner
Part IV: Therapeutics and Engineered Systems
14. Evaluating the Spatial and Temporal Protein Production in Neural Tissue Engineering Constructs In Vitro
Caroline P. Addington, Christine Pauken, and Sarah E. Stabenfeldt
15. Cultivation and Imaging of Astrocytes on Protein-Coated Fluorescent Topographies Constructed from Aligned PLLA Electrospun Fibers
Jonathan M. Zuidema, María C. Hyzinski-García, Alexander A. Mongin, and Ryan J. Gilbert
16. Engineered Microdevices to Study and Manipulate Neural Stem Cell Chemotaxis
Hui Xu and Sarah C. Heilshorn
17. Hydrolytically Degradable Polyethylene Glycol (PEG) Hydrogel: Synthesis, Gel Formation, and Characterization
Silviya Petrova Zustiak.