1. Overview

Mihaela Gadjeva

2. Evaluation of Complement Function by ELISA

Anja Roos and Jorgen Wieslander

3. Classical Complement Pathway Component C1q - Purification of Human C1q, Isolation of C1q Collagen-like and Globular Head Fragments and Production of Recombinant C1q - Derivatives. Functional Characterization.

Mihaela Kojouharova

4. Classical Complement Pathway Components C1r and C1s - Purification from Human Serum and in Recombinant Form and Functional Characterization

Véronique Rossi, Isabelle Bally, Monique Lacroix, Gérard J Arlaud, and Nicole M Thielens

5. Expression and Purification Methods for the Production of Recombinant Human Complement Component C2

Angela W. Norton, Paolo G.V. Martini, Lynette C. Cook , Scott Alderucci , Dianna M. Lundberg, Susan M. Fish , Charles Bedard³, John Gill, Arthur O. Tzianabos, and Michael F. Concino

6. Purification and Characterisation of human and Mouse Complement C3

Marieta M Ruseva and Meike Heurich

7. Purification of Human Complement Protein C5

Lars Sottrup-Jensen and Gregers Rom Andersen

8. Quantification of Complement C5b-9 Binding to Cells by Flow Cytometry

Oren Moskovich and Zvi Fishelson

9. Challenges and Opportunities in Fractionation of Recombinant Human Mannan-binding Lectin

Thomas Vorup-Jensen

10. Genotyping of FCN and MBL2 Polymorphisms using Pyrosequencing

Lea Munthe-Fog, Hans Ole Madsen, and Peter Garred

11. Assay for Estimation of the Functional Activity of the Mannan Binding Lectin Pathway of the Complement System

Troels R. Kjaer and Steffen Thiel

12. Purification, Measurement of Concentration, and Functional Complement Assay of Human Ficolins

Misao Matsushita, David Kilpatrick, Hiroshi Shiraki, Yu Liu, Koichiro Tateishi, Mitsushi Tsujimura, Yuichi Endo, Teizo Fujita

13. Purification and Functional Analysis of Human Properdin

Joseph O`Flynn, Maria C. Faber Krol, Mohamed R. Daha, Cees van Kooten

14. Purification and Functional Characterization of C4b-binding Protein (C4BP)

Frida C. Mohlin and Anna M. Blom

15. Purification and Functional Characterization of Factor I

Sara C. Nilsson and Anna M. Blom

16. C1 inhibitor - Quantification and Purification

Lilian Varga and József Dobó

17. Purification, Quantification and Functional Analysis of Complement Factor H

Mihály Józsi and Seppo Meri

19. Functional Evaluation of Factor H genetic and Acquired Abnormalities; Application for Atypical Hemolytic Uremic Syndrome (aHUS)

Lubka T. Roumenina, Roxane Roquigny, Caroline Blanc, Nelly Poulain, Stephanie Ngo, Marie-Agnes Dragon-Durey, and Veronique Fremeaux-Bacchi

20. Anti-Factor H Auto Antibodies Assay

Marie-Agnès Dragon-Durey, Caroline Blanc, Lubka Roumenina, Nelly Poulain Poulain, Stéphanie Ngo, Pauline Bordereau, Véronique Frémeaux-Bacchi

21. Purification of Native Surfactant Protein SP-A from Pooled Amniotic Fluid and Bronchoalveolar Lavage

Najmunisa Karbani, Eswari Dodagatta-Marri, Asif S. Qaseem, Priyaa Madhukaran, Patrick Waters, Anthony G. Tsolaki, Taruna Madan, and Uday Kishore

22. Purification of Surfactant Protein D (SP- D) from Pooled Amniotic Fluid and Bronchoalveolar Lavage

Eswari Dodagatta-Marri, Asif Qaseem, Najmunisa Karbani, Anthony George Tsolaki, Patrick Waters, Taruna Madan, and Uday Kishore

23. Functional Analysis of C5a Effector Responses In Vitro and In Vivo

Christian M. Karsten, Yves Laumonnier, and Jörg Köhl