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Calpain

Calpain


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About the Book

Preface...
Table of Contents...
Contributing Authors...

Part I Production and Purification of Calpains

1. Single-Step Purification of Calpain-1, Calpain-2, and Calpastatin using Anion Exchange Chromatography
Ashim Kumar Biswas and Samarth Tandon

2. Bacterial Expression and Purification of Calpains
Christian-Scott E. McCartney and Peter L. Davies

Part II Determination of Calpain Localization, Expression, and Activity

3. Casein Zymography for Analysis of Calpain-1 and Calpain-2 Activity
Ashim Kumar Biswas and Samarth Tandon

4. FRET-Based Assays to Determine Calpain Activity
Christian-Scott E. McCartney and Peter L. Davies

5. In Vivo Monitoring of Calpain Activity by Forster Resonance Energy Transfer
William Lostal, Daniel Stockholm, and Isabelle Richard

6. Determining Temporal and Spatial Expression of Calpains in Amphibians
Anna Charalambous, Ioanna Antoniades, Neophytos Christodoulou, Sara Zanardelli, and Paris A. Skourides

7. Immunohistochemical Localization of Calpains in the Amphibian Xenopus laevis
Jean-Marie Exbrayat, Elara N. Moudilou, and Claire Brun

8. Expression and Activity of Calpain A in Drosophila melanogaster
Maira Cardoso, Danielle Oliveira, and Helena Araujo

9. Use of the β-Glucoronidase Reporter System (GUS) to Localize Promoter Activities of the Endogenous Plant Calpain DEFECTIVE KERNEL1 (DEK1)
Zhe Liang and Hilde-Gunn Opsahl-Sorteberg

Part III Identification of Calpain Substrates and Calpain-Activated Protein Functions

10. LabCaS for Ranking Potential Calpain Substrate Cleavage Sites from Amino Acid Sequence
Yong-Xian Fan, Xiaoyong Pan, Yang Zhang, and Hong-Bin Shen

11. CalCleaveMKL: A Tool for Calpain Cleavage Prediction
David A. duVerle and Hiroshi Mamitsuka

12. Identification of Calpain-Activated protein Functions
Maria del Carmen Lafita-Navaro and Maralice Conacci-Sorrell

Part IV Interrogation of Calpastatin, The Endogenous Calpain Inhibitor

13. Production and Purification of Recombinant Calpastatin
R. De Tullio and M. Averna

14. Isolation of Endogenous Calpastatin
R. De Tullio and M. Averna

15. Immunoblotting for Calpastatin Expression
M. Averna and R. De Tullio

Part V Manipulation of Calpain Expression

16. Experimental Manipulation of Calpain Activity In Vitro
Seiichi Kitagawa

17. In Vivo Calpain Knockdown using Delivery of siRNA
Xiaoqian Liu and Guochang Hu

18. In Vivo Expression of Mutant Calpains in the Eye using Lentivirus
Katherine J. Wert and Vinit B. Mahajan

19. Methods of Calpain Inhibition to Determine the Role of Calpains in Embryo Development in Amphibians
Ioanna Antoniades, Anna Charalambous, Neophytos Christodoulou, Sara Zanardelli, and Paris A. Skourides

20. Genetic Models of Calpain Deficiency and Ectopic Expression
Yan Gao, Christine Hall, James MacLeod, and Peter A. Greer


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Product Details
  • ISBN-13: 9781493989874
  • Publisher: Springer
  • Publisher Imprint: Springer
  • Height: 254 mm
  • No of Pages: 276
  • Series Title: Methods in Molecular Biology
  • Sub Title: Methods and Protocols
  • Width: 178 mm
  • ISBN-10: 1493989871
  • Publisher Date: 08 Jan 2019
  • Binding: Hardback
  • Language: English
  • Returnable: Y
  • Spine Width: 18 mm
  • Weight: 770 gr


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