Part I: Intracellular Ca²⁺- Measurements and Cation Interactions with EF-Hand Proteins

1. Measurement of Intracellular Ca²⁺ Concentration in Single Cells using Ratiometric Calcium Dyes

Sonal Srikanth and Yousang Gwack

2. Divers Models of Divalent Cation Interaction to Calcium-binding Proteins: Techniques and Anthology

Jos A. Cox

3. Probing Ca²⁺ Binding Capability of Viral Proteins with the EF-hand Motif by Grafting Approach

Yubin Zhou, Shenghui Xue, Yanyi Chen, Jenny J. Yang

Part II: Expression, Purification, Biochemical, and Structural Analyses and Cellular Localization

4. New Aspects of Calmodulin-calmodulin Binding Domains Recognition.

Emilie Audran, Rania Dagher, Sophie Gioria, Marie-Claude Kilhoffer and Jacques Haiech

5. Purification and Characterization of the Human Cysteine-rich S100A3 Protein and its Pseudo Citrullinated Forms Expressed in Insect Cells

Kenji Kizawa, Masaki Unno, Hidenari Takahara, Claus W. Heizmann

6. X-ray Structural Analysis of S100 Proteins

Dr. Günter Fritz

7. Purification and Stable Isotope Labeling of the Calcium and Integrin Binding Protein 1 for Structural and Functional NMR Studies

Hao Huang and Hans J. Vogel

8. Analysis of Calcium-induced Conformational Changes in Calcium-binding Allergens and Quantitative Determination of their IgE Binding Properties

Nuria Parody, Miguel Ángel Fuertes, Carlos Alonso, Yago Pico de Coaña

9. Longistatin, an EF-hand Ca²⁺-binding Protein From Vector Tick: Identification, Purification, and Characterization

Anisuzzaman, M. Khyrul Islam, M. Abdul Alim and Naotoshi Tsuji

10. Super-Resolution Microscopy of the Neuronal Calcium-Binding Proteins Calneuron-1 and Caldendrin

Johannes Hradsky, Marina Mikhaylova, Anna Karpova, Michael R. Kreutz, and Werner Zuschratter

Part III: Interaction of Ca²⁺-Binding Proteins with Targets and Receptors/RAGE

11. NMR Studies of the Interaction of Calmodulin with IQ Motif Peptides

Steven M. Damo, Michael D. Feldkamp, Benjamin Chagot, Walter J. Chazin

12. Biochemical and Immunological Detection of Physical Interactions Between Penta-EF-hand Protein ALG-2 and Its Binding Partners

Kanae Osugi, Hideki Shibata and Masatoshi Maki

13. Measuring binding of S100 proteins to RAGE by Surface Plasmon Resonance

Estelle Leclerc

14. Phage Display Selection of Peptides that Target Calcium-Binding Proteins

Stefan W. Vetter

Part IV: RAGE-Meditated CELL Signalling and Pathology

15. RAGE-mediated Cell Signalling

Ari Rouhiainen, Juha Kuja-Panula, Sarka Tumova, Heikki Rauvala,

16. RAGE Splicing Variants in Mammals

Katharina Anna Sterenczak, Ingo Nolte, and Hugo Murua Escobar

Part V: Ca²⁺-Binding Proteins in Human Diseases and As Drug Targets

17. Screening for Novel Calcium Binding Proteins that Regulate Cardiac Hypertrophy: CIB1 as an Example

Joerg Heineke, MD

18. In Vivo Screening of S100B Inhibitors for Melanoma Therapy

Danna B. Zimmer, Rena G. Lapidus and David J. Weber

19. Arrayed Primer Extension Microarray for the Analysis of Genes Associated with Congenital Stationary Night Blindness

Kadri Vaidla, Janne Üksti, Christina Zeitz, Eneli Oitmaa

20. Nesfatin-1: Its Role in the Diagnosis and Treatment of Obesity and Some Psychiatric Disorders

Hiroyuki Shimizu and Masatomo Mori

Part VI: Cell Line Authentication

21. Human Cell Line Authentication: The Critical First Step in any Project Using Human Cell Lines

Robert S. McLaren, Yvonne Reid, and Douglas R. Storts

Part VII: Biomarkers, Diagnostic, C