Part I: In Vitro Culture of Bone Cells
1. Isolation and Generation of Osteoclasts
Nadia Rucci, Alberta Zallone, and Anna Teti
2. Isolation and Generation of Osteoblasts
Inês P Perpétuo, Lucie E Bourne, and Isabel R Orriss
3. Isolation, Purification, Generation, and Culture of Osteocytes Jonathan H Gooi, Ling Yeong Chia, Christina Vrahnas, and Natalie A Sims
4. Isolation, Differentiation, and Characterisation of Human Bone Marrow Stem Cells In Vitro and In Vivo
Janos Kanczler, Rahul S. Tare, Peter D. Mitchell, Patrick Stumpf, Timothy J. Noble, Cameron Black, and Richard O.C. Oreffo
5. 2d and 3d In Vitro Co-Culture For Cancer and Bone Cell Interaction Studies
Silvia Marino, Ryan T. Bishop, Daniëlle De Ridder, Jesus Delgado-Calle, and Michaela R Reagan
6. Using Cell and Organ Culture Models to Analyse Responses of Bone Cells to Mechanical Stimulation
Soraia P Caetano-Silva, Astrid Novicky, Behzad Javaheri, Simon Cf Rawlinson, and Andrew A Pitsillides
Part II: Ex Vivo Culture of Bone Cells
7. The ex vivo Organ Culture of Bone
Katherine A. Staines, Genevieve Brown, and Colin Farquharson
8. Ex-Vivo Model Systems of Cancer-bone Cell Interactions
Erdjan Salih
9. Mechanical Properties of Bone ex vivo Simon R. Goodyear and Richard M. Aspden
Part III: Ex Vivo Culture of Bone Cells
10. Analysis of Signalling Pathways by Western Blotting and Immunoprecipitation Silvia Marino and Aymen I. Idris
11. Analysis of Transcriptional Regulation in Bone Cells Huilin Jin, Antonia Sophocleous, Asim Azfer, and Stuart H Ralston
12. Analysis of mRNA, miRNA, and DNA in bone cells by RT-qPCR and In situ Hybridization
Brice Moukengue, Jérôme Amiaud, Camille Jacques, Céline Charrier, Benjamin Ory, and Francois Lamoureux
Part IV: In Vivo Models of Bone
13. Ovariectomy/Orchiectomy in Rodents Antonia Sophocleous and Aymen I. Idris
14. In Vivo Models of Rheumatoid Arthritis
Bettina Grötsch, Aline Bozec and Georg Schett 15. Osteoarthritis Mouse Model of Destabilisation of the Medial Meniscus Antonia Sophocleous and Carmen Huesa
16. Models of Prostate Cancer Bone Metastasis Sun Hee Park, Matthew Robert Eber, andYusuke Shiozawa
17. Animal Models of Breast Cancer Bone Metastasis
Claudia Tulotta, Arwin Groenewoud, B. Ewa Snaar-Jagalska, and Penelope Ottewell
18. Murine Models of Bone Sarcomas
Camille Jacques, Nathalie Renema, Benjamin Ory, Carl R. Walkley, Agi E. Grigoriadis, and Dominique Heymann
19. Mouse Models of Melanoma Bone Metastasis
Özge Uluçkan
20. Animal Models of Multiple Myeloma
Sam W.Z. Olechnowicz and Claire M. Edwards
21. The Calvarial Injection Assay
Robert J. van 't Hof
22. In Vivo Models of Mechanical Loading Behzad Javaheri, Nathalie Bravenboer, Astrid D. Bakker, Albert van der Veen, Roberto Lopes de Souza, Leanne Saxon, and Andrew A. Pitsillides
23. Animal Models for the Study of Bone Derived Pain
Austen L. Thompson, Tally M. Largent-Milnes, and Todd W. Vanderah
Part V: Microscopical and Imaging Techniques
24. Histomorphometry in Rodents
Reinhold G. Erben and Martin Glösmann
25. Immunostaining of Skeletal Tissues
Anke J. Roelofs and Cosimo De Bari
