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Bcl-2 Family Proteins

Bcl-2 Family Proteins


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About the Book

Preface...

Table of Contents...

Contributing Authors...


1. Overview of BCL-2 Family Proteins and Therapeutic Potentials
Jason D. Huska, Heather M. Lamb, and J. Marie Hardwick

2. Database and Bioinformatic Analysis of BCL-2 Family Proteins and BH3-Only Proteins
Abdel Aouacheria, Vincent Navratil, and Christophe Combet

3. Application of Mito-Priming to Generate BCL-2 Addicted Cells
Jonathan Lopez and Stephen W.G. Tait

4. BH3 Profiling: A Functional Assay to Measure Apoptotic Priming and Dependencies
Cameron Fraser, Jeremy Ryan, and Kristopher Sarosiek

5. Flow Cytometry-Based Detection and Analysis of BCL-2 Family Proteins and Mitochondrial Outer Membrane Permeabilization (MOMP)
Linsey M. Ludwig, Katrina L. Maxcy, and James L. LaBelle

6. Investigating BCL-2 Family Protein Interactions in Yeast
Stéphen Manon

7. Liposomal Permeabilization Assay to Study the Functional Interaction of the BCL-2 Family
Denis E. Reyna and Evripidis Gavathiotis

8. Mitochondrial Isolation, Gel Filtration, and Real-Time Monitoring of MOMP
Mark P.A. Luna-Vargas, Jarvier N. Mohammed, and Jerry E. Chipuk

9. Photocrosslinking Approach to Investigate Protein Interactions in the BCL-2 Family
Jialing Lin, Arthur E. Johnson, and Zhi Zhang

10. Assessment of Dynamic BCL-2 Protein Shuttling between Outer Mitochondrial Membrane and Cytosol
Joachim Lauterwasser, Franziska Fimm-Todt, and Frank Edlich

11. Quantification of BCL-2 Family Members by Flow Cytometry
Morey L. Smith and Stephen K. Tahir

12. Methods to Probe Calcium Regulation by BCL-2 Family Members
Marcos A. Carpio and Samuel G. Katz

13. Methods to Probe Conformational Activation and Mitochondrial Activity of Proapoptotic BAK
Geetika Singh and Tudor Moldoveanu

14. Probing BAK and BAX Activation and Pore Assembly with Cytochrome c Release, Limited Proteolysis and Oxidant-Induced Linkage
Sweta Iyer, Rachel T. Uren, and Ruth M. Kluck

15. BCL-2 Protein Family Interaction Analysis by Nuclear Magnetic Resonance Spectroscopy
Thomas P. Garner and Evripidis Gavathiotis

16. Reconstitution and Characterization of BCL-2 Family Proteins in Lipid Bilayer Nanodiscs
Yong Yao and Francesca M. Marassi

17. Cryo-Electron Microscopy to Study Bax Pores and MOMP
Tomomi Kuwana

18. CW EPR and DEER Methods to Determine BCL-2 Family Protein Structure and Interactions: Application of Site-Directed Spin Labeling to BAK Apoptotic Pores
Tirtha Mandal, Eric J. Hustedt, Likai Song, and Kyoung Joon Oh

19. Rapid Imaging of BCL-2 Family Interactions in Live Cells using FLIM-FRET
Elizabeth J. Osterlund, Nehad Hirmiz, Christian Tardiff, and David W. Andrews

20. Quantification of the Interactions between BCL-2 Proteins by Fluorescence Correlation Spectroscopy
Fabronia Murad and Ana J. Garcia-Saez

21. Isolation of Synthetic Antibodies against BCL-2-Associated X Protein (BAX)
Zhou Dai and Jonathan R. Lai


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Product Details
  • ISBN-13: 9781493988600
  • Publisher: Humana Press
  • Publisher Imprint: Humana Press
  • Height: 254 mm
  • No of Pages: 360
  • Series Title: Methods in Molecular Biology
  • Sub Title: Methods and Protocols
  • Width: 178 mm
  • ISBN-10: 1493988603
  • Publisher Date: 13 Nov 2018
  • Binding: Hardback
  • Language: English
  • Returnable: Y
  • Spine Width: 22 mm
  • Weight: 911 gr


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