About the Book
Part I: General Introduction
1. Defense and counter-defense in the RNAi-based antiviral immune system in insects
Joël T. van Mierlo, Koen W.R. van Cleef, and Ronald P. van Rij
2. RNAi and cellular miRNAs in infections by mammalian viruses
Joost Haasnoot and Ben Berkhout
3. Viral miRNAs
Karlie Plaisance-Bonstaff, and Rolf Renne
4. Progress in RNAi-based antiviral therapeutics
Jiehua Zhou and John J. Rossi
5. Chemical modification of small interfering RNA
Jesper B. Bramsen and Jørgen Kjems
Part II: Viral small RNAs
6. Viral small RNA cloning and sequencing
Valérie Gausson and Maria-Carla Saleh
7. Visitor, an informatic pipeline for analysis of viral siRNA sequencing datasets
Christophe Antoniewski
8. Computational prediction of viral miRNAs
Adam Grundhoff
9. Detection of Viral microRNAs by Northern Blot Analysis
Lydia V. McClure, Yao-Tang Lin, and Christopher S. Sullivan
10. Detection of viral microRNA with S1 Nuclease Protection assay
Matthias John and Sébastien Pfeffer
11. Characterization of RISC associated adenoviral small RNAs
Ning Xu and Göran Akusjärvi
Part III: RNAi-based antiviral defense
12. Identification of Viral Suppressors of RNAi by a Reporter Assay in Drosophila S2 Cell Culture
Koen W.R. van Cleef, Joël T van Mierlo, Marius van den Beek, and Ronald P. van Rij
13. Dicer assay in Drosophila S2 cell extract
Baojun Yang and Hongwei Li
14. Slicer activity in Drosophila melanogaster S2 extract
Arabinda Nayak and Raul Andino
15. Gel Mobility Shift Assays for RNA-binding viral RNAi suppressors
Tibor Csorba and József Burgyán
16. dsRNA uptake in adult Drosophila
Benjamin Obadia and Maria-Carla Saleh
Part IV: Antiviral RNAi therapy
17. Design of small interfering RNAs for antiviral applications
Diana Rothe, Erik J. Wade, and Jens Kurreck
18. RNAi inducing lentiviral vectors for anti-HIV-1 gene therapy
Ying Poi Liu, Jan-Tinus Westerink, Olivier ter Brake, and Ben Berkhout
19. Production of multicopy shRNA lentiviral vectors for antiviral therapy
Scot D. Henry, Quiwei Pan, and Luc J.W. van der Laan
20. Intranasal delivery of antiviral siRNA
Sailen Barik
21. Antibody mediated delivery of siRNAs for anti-HIV therapy
Sang-Soo Kim, Sandesh Subramanya, Dan Peer, Motomu Shimaoka, and Premlata Shankar
22. Aptamer-targeted
