Preface...
Table of Contents...
Contributing Authors...
1. Production and Crystallization of Full-Length Human AMP-Activated Protein Kinase (a1b1y1)
Julia A. Hubbard, Bing Xiao, and Jon R. Wilson
2. Visualizing AMPK Drug Binding Sites through Crystallization of Full Length Phosphorylated a2b1y1 Heterotrimer
Christopher G. Langendorf, Jonathan S. Oakhill, and Bruce E. Kemp
3. Biophysical Interactions of Direct AMPK Activators
Ravi G. Kurumbail, Graham M. West, Venkatasubramanian Dharmarajan, Kris A. Borzilleri, Jane M. Withka, Jessica Ward, Allan R. Reyes, Francis Rajamohan, Patrick R. Griffin, and Matthew F. Calabrese
4. Biochemical Measurement of Glycogen: Method to Investigate the AMPK-Glycogen Relationship
Elite Possik and Arnim Pause
5. Cell-Free Assays to Measure Effects of Regulatory Ligands on AMPK
Fiona A. Fyffe, Simon A. Hawley, Alexander Gray, and D. Grahame Hardie
6. Applications of NMR and ITC for the Study of the Kinetics of Carbohydrate Binding by AMPK β-Subunit Carbohydrate Binding Modules
Paul R. Gooley, Ann Koay, and Jesse I. Mobbs
7. Bioinformatics Approach to Identify Novel AMPK Targets
Brendan Gongol, Traci Marin, David A. Johnson, and John Y-J. Shyy
8. Studying AMPK in an Evolutionary Context
Arpit Jain, Valentin Roustan, Wolfram Weckwerth, and Ingo Ebersberger
9. AMPK Protein Interaction Analyses by Yeast Two-Hybrid
Pascual Sanz, Rosa Viana, and Maria Adelaida Garcia-Gimeno
10. Transient Expression of AMPK Heterotrimer Complexes in Mammalian Cells
Jonathan S. Oakhill, John W. Scott, and Toby A. Dite
11. Knockdown of Human AMPK using the CRISPR-Cas9 Genome-Editing System
Adrien Grenier, Pierre Sujibert, Séverine Olivier, Hélène Guermouche, Johanna Mondésir, Olivier Kosmider, Benoit Viollet, and Jérôme Tamburini
12. Compound C/Dorsomorphin - Its Use and Misuse as an AMPK Inhibitor
Biplab Dasgupta and William Seibel
13. Identifying the Heterotrimeric Complex Stoichiometry of AMPK in Skeletal Muscle by Immunoprecipitation
Jesper B. Birk and Jørgen F. P. Wojtaszewski
14. Kinase Activity Determination of Specific AMPK Complexes/Heterotrimers in Skeletal Muscle
Jesper B. Birk and Jørgen F. P. Wojtaszewski
15. Determination of Adenine Nucleotide Concentrations in Cells and Tissues by High Performance Liquid Chromatography
Noemí García-Tardón and Bruno Guigas
16. Intact Cell Assays to Monitor AMPK and Determine the Contribution of the AMP-Binding or ADaM Sites to Activation
Simon A. Hawley, Fiona A. Fyffe, Fiona M. Russell, Graeme J. Gowans, and D. Grahame Hardie
17. Cellular Application of Genetically-Encoded Sensors and Impeders of AMPK
Takafumi Miyamoto, Elmer Rho, Allen Kim, and Takanari Inoue
18. Assessing Mitochondrial Bioenergetics by Respirometry in Cells or Isolated Organelles
Guillaume Vial and Bruno Guigas
19. Study of AMPK Regulated Metabolic Fluxes in Neurons using the Seahorse XFe Analyzer
Claudia Marinangeli, Jérome Kluza, Philippe Marchetti, Luc Buée, and Valérie Vingtdeux
20. Investigating the Role of AMPK in Inflammation
Sarah J. Mancini and Ian P. Salt
21. Studying the Role of AMPK in Cardiac Hypertrophy and Protein SynthesisFlorence Mailleux, Christophe Beauloye, Jean-Luc Balligand, Sandrine Horman, and Luc Bertrand
22. Assessment of AMPK-Stimulated Cellular Long-Chain Fatty Acid and Glucose Uptake
Joost J.F.P. Luiken, Dietbert Neumann, Jan F.C. Glatz, Will A. Coumans, Dipanjan Chanda, and Miranda Nabben
23. Measurement of AMPK-Induced Inhibition of Lipid Synthesis Flux in Cultured CellsMarc Foretz and Benoit Viollet
24. Studying the Role of AMPK in Autophagy
Sarah Krieg, Bernhard Lüscher, Jörg Vervoorts, and Marc Dohmen
25. Determining AMPK Activation via the Lysosomal v-
