Preface. Complex Biologics: Engineering the Tools and the Hosts. M. Cristina Vega, Francisco J. Fernández and Imre Berger
Section 1: Introduction
1. Protein complex production from the drug discovery standpoint; Ismail Moarefi
Choose a suitable expression host: a survey of available protein production platforms; Francisco J. Fernández and M. Cristina Vega
Section 2: Prokaryotic expression hosts
3. ACEMBL Tool-Kits for High-Throughput Multigene Delivery and Expression in Prokaryotic and Eukaryotic Hosts; Yan Nie, Maxime Chaillet, Christian Becke, Matthias Haffke, Martin Pelosse, Daniel Fitzgerald, Ian Collinson, Christiane Schaffitzel and Imre Berger
4. Complex reconstitution and characterization by combining co-expression techniques in Escherichia coli with high-throughput; Renaud Vincentelli and Christoph Romier
5. Membrane protein production in E. coli for applications in drug discovery; Harm Jan (Arjan) Snijder and Jonna Hakulinen
6. Cell-free Synthesis of Macromolecular Complexes; Matthieu Botte, Aurélien Deniaud and Christiane Schaffitzel
7. A Bacillus megaterium system for the production of recombinant proteins and protein complexes; Rebekka Biedendieck
8. Protein complex production in alternative prokaryotic hosts; Sara Gómez, Miguel López-Estepa, Francisco J. Fernández and M. Cristina Vega
Section 3: Lower Eukaryotic expression hosts
9. Production of Protein Complexes in Non-methylotrophic and Methylotrophic Yeasts; Francisco J. Fernandez, Miguel López-Estepa, Javier Querol-García and M. Cristina Vega
10. Leishmania tarentolae for the Production of Multi-Subunit Complexes; Tomoaki Niimi
11. Alternative Eukaryotic Expression Systems for the Production of Proteins and Protein Complexes; Sara Gómez, Miguel López-Estepa, Francisco J. Fernández, Teresa Suárez and M. Cristina Vega
Section 4: Higher Eukaryotic expression hosts
12. Fundamentals of baculovirus expression and applications; Tom A. Kost and Christopher W. Kemp
13. The MultiBac Baculovirus / Insect Cell Expression Vector System for Producing Complex Protein Biologics; Duygu Sari, Kapil Gupta, Deepak Balaji Govinda Raj, Alice Aubert, Petra Dmcova, Frederic Garzoni, Daniel Fitzgerald and Imre Berger
14. Fundamentals of expression in mammalian cells; Michael R. Dyson
15. Assembling multi-subunit complexes using mammalian expression; Bahar Baser and Joop van den Heuvel