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Advanced Protocols for Animal Transgenesis

Advanced Protocols for Animal Transgenesis


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About the Book

Karen S. Canady: Patent and licensing issues in transgenic technology.- Lluís Montoliu: Global Resources: Including Gene Trapped ES Cell Clones: Is Your Gene Already Knocked Out?.- Eduardo Moltó, Cristina Vicente-García and Lluís Montoliu: Designing Transgenes for Optimal Expression.- Thomas L. Saunders: Gene Targeting Vector Design for Embryonic Stem Cell Modifications.- Thomas J. Fielder and Lluis Montoliu: Transgenic Production Benchmarks.- Katja Becker and Boris Jerchow: Generation of Transgenic Mice by Pronuclear Microinjection.- Séverine Ménoret, Séverine Remy, et al: Generation of Transgenic Rats using Microinjection of Plasmid DNA or Lentiviral vectors.- Almudena Fernández, Diego Muñoz and Lluís Montoliu: Generation of Transgenic Animals by Use of YACs.- Michael G. Zeidler, Margaret L. Van Keuren, and Thomas L. Saunders: BAC Transgenes, DNA Purification, and Transgenic Mouse Production.- Carlos Lois: Generation of Transgenic Animals with Lentiviral Vectors.- Aron Geurts, Lajos Mates, Darius Balciunas: Vertebrate Transgenesis by Transposition.- Karen M. Chapman et al.: Rat Spermatogonial Stem Cell Mediated Gene Transfer.- Sayaka Wakayama, Nguyen Van Thuan and Teruhiko Wakayama: Mouse Cloning by Nuclear Transfer.- Elizabeth D. Hughes and Thomas L. Saunders: Gene Targeting in Embryonic Stem Cells.- Wojtek Auerbach and Anna B. Auerbach: The Importance of Mouse ES Cell Line Selection.- Marina Gertsenstein: Tetraploid Complementation Assay.- Elizabeth Williams et al.: Combining ES cells with Embryos.- Kristina Nagy and Jennifer Nichols: Derivation of Murine ES Cell Lines.- Ping Li et al.: Rat Embryonic Stem Cell Derivation and Propagation.- Han Li et al.: Induced pluripotency: generation of iPS cells from mouse embryonic fibroblasts.- Anna B. Auerbach, Peter J. Romanienko, and Willie H. Mark: The Preparation and Analysis of DNA for Use in Transgenic Technology.- Karen Brennan: Colony Management.- B. Pintado and J Hourcade: Cryopreservation.- Shirley Pease: Shipment of Mice and Embryos.- J.M. Sztein, R.J. Kastenmayer, K.A. Perdue: Pathogen Free Mouse Rederivation by IVF, Natural Mating and Hysterectomy.- Jan Parker-Thornburg: Refinement, Reduction and Replacement.


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Product Details
  • ISBN-13: 9783662519431
  • Publisher: Springer
  • Publisher Imprint: Springer
  • Edition: Softcover reprint of the original 1st ed. 2011
  • Language: English
  • Returnable: Y
  • Spine Width: 35 mm
  • Weight: 1223 gr
  • ISBN-10: 3662519437
  • Publisher Date: 23 Aug 2016
  • Binding: Paperback
  • Height: 254 mm
  • No of Pages: 669
  • Series Title: Springer Protocols Handbooks
  • Sub Title: An Istt Manual
  • Width: 178 mm


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Advanced Protocols for Animal Transgenesis
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