Preface...

Table of Contents...

Contributing Authors...

Part I Introduction

1. Vitamin B3 in Health and Disease: Toward the Second Century of Discovery

Myron K. Jacobson and Elaine L. Jacobson

Part II ADPribose Binding Domains as Tools

2. Monitoring Poly(ADP-Ribosyl)ation in Response to DNA Damage in Live Cells Using Fluorescently-Tagged Macrodomains

Rebecca Smith and Gyula Timinszky

3. In Vitro Techniques for ADP-Ribosylated Substrate Identification

Giovanna Grimaldi, Giulianan Catara, Carmen Valente, and Daniela Corda

4. Assessment of Intracellular Auto-Modification Levels of ARTD10 using Mono-ADP-Ribose-Specific Macrodomains 2 and 3 of Murine Artd8

Mareike Bütepage, Sarah Krieg, laura Eckei, Jinyu Li, Giulia Rossetti, Patricia Verheugd, and Bernhard Lüscher

5. Biochemical and Biophysical Assays of PAR-WWE Domain Interactions and Production of iso-ADPr for PAR Binding Analysis

Zhizhi Wang and Wenqing Xu

Part III Enzymatic Assays of NAD+ Utilizing Enzymes

6. Assays for NAD-Dependent Reactions and NAD Metabolites

Michael B. Schultz, Yuancheng Lu, Nady Braidy, and David A Sinclair

7. Generating Protein-Linked and Protein-Free Mono-, Oligo-, and Poly(ADP-Ribose) In Vitro

Ken Y. Lin, Dan Huang, and W. Lee Kraus

8. Methods to Study TCDD-Inducible Poly-ADP-Ribose Polymerase (TIPARP) Mono-ADP-Ribosyltransferase Activity

David Hutin, Giulia Grimaldi, and Jason Matthews

9. Dictyostelium as a Model to Assess Site-Specific ADP-Ribosylation Events

Anna-Lena Kolb, Duen-Wei Hsu, Ana B.A. Wallis, Seiji Ura, Alina Rakhimova, Catherine J. Pears, and Nicholas D. Lakin

10. Mono-ADP-Ribosylation Catalyzed by Arginine-Specific ADP-Ribosyltransferases

Linda A. Stevens and Joel Moss

11. Monitoring Expression and Enzyme Activity of Ecto-ARTCs

Stephan Menzel, Sahil Abriouch, Peter Bannas, Friedrich Haag, and Friedrich Koch-Nolte

12. ADP-Ribosyl-Acceptor Hydrolase Activities Catalyzed by the ARH Family of Proteins

Masato Mashimo and Joel Moss

13. Mono-ADP-Ribosylhydrolase Assays

14. Hydrolysis of ADP-Ribosylation by Macrodomains

Melanija Posavec Marjanovic, Gytis Jankevicius, and Ivan Ahel

15. HPLC-Based Enzyme Assays for Sirtuins

Jun Young Hong, Xiaoyu Zhang, and Hening Lin

Part IV Small Molecule Screening Assays of NAD+ Utilizing Enzymes

16. Small Molecule Screening Assay for Mono-ADP-Ribosyltransferases

Haikarainen Teemu, Murphy Sudarshan, Maksimainen Mirko, and Lari Lehtiö

17. ¬A Simple, Sensitive, and Generalizable Plate Assay for Screening PARP Inhibitors

Ilsa T. Kirby, Rory K. Morgan, and Michael S. Cohen

Part V Mass Spectrometry Techniques for Detection of Mono-ADP-Ribosylation

18. Non-Localized Searching of HCD Data for Fast and Sensitive Identification of ADP-Ribosylated Peptides

Thomas Colby, Juan José Bonfiglio, and Ivan Matic

Robert Lyle Mcpherson, Shao-En Ong, and Anthony K.L. Leung

Part VI Mono-ADP-Ribose and Disease

20. Detection of ADP-Ribosylating Bacterial Toxins

Chen Chen and Joseph T. Barbieri

21. Preparation of Recombinant Alphaviruses for Functional Studies of ADP-Ribosylation

Rachy Abraham, Robert L. McPherson, Easwaran Sreekumar, Anthony K.L. Leung, and Diane E. Griffin

22. Monitoring the Sensitivity of T Cell Populations towards NAD+ Released during Cell Preparation

Björn Rissiek, Marco Lukowiak, Friedrich Ha