About the Book
1. 1D4 - A Versatile Epitope Tag for the Purification and Characterization of Expressed Membrane and Soluble Proteins
Laurie L. Molday and Robert S. Molday
2. Affinity Purification of Heme-Tagged Proteins
Wesley B. Asher and Kara L. Bren
3. Purification of a Recombinant Protein with Cellulose-Binding Module 3 as the Affinity Tag
Dongmei Wang and Jiong Hong
4. Purification of E. coli Proteins Using a Self-Cleaving Chitin-Binding Affinity Tag
Michael J. Coolbaugh and David W. Wood
5. Simplified Protein Purification Using an Autoprocessing, Inducible Enzyme Tag
Aimee Shen
6. SUMO as a Solubility Tag and in vivo Cleavage of SUMO Fusion Proteins with Ulp1
Dennis Kuo, Minghua Nie, and Albert J. Courey
7. Rescuing Aggregation-Prone Proteins in Escherichia coli with a Dual His6-MBP Tag
Danielle Needle and David S. Waugh
8. Expression, Purification, and Immobilization of Recombinant Tamavidin 2 Fusion Proteins
Yoshimitsu Takakura, Naomi Oka, and Masako Tsunashima
9. Use of Tandem Affinity Chromatography for Purification of Cannabinoid Receptor CB2
Silvia C. Locatelli-Hoops and Alexei A. Yeliseev
10. Detection of Protein-Protein Interactions Using Tandem Affinity Purification
Ian Goodfellow and Dalan Bailey
11. An Improved in vivo Biotinylation Strategy Combined with FLAG and Antibody Based Approaches for Affinity Purification of Protein Complexes in Mouse Embryonic Stem Cells
Francesco Faiola, Arven Saunders, Baoyen Dang, and Jianlong Wang
12. Purification of Recombinant Proteins with a Multifunctional GFP Tag
Takashi Murayama and Takuya Kobayashi
13. Targeted Purification of SnAvi-Tagged Proteins
Ursula Schäffer, Ralf Baumeister, and Ekkehard Schulze
14. An Efficient Fluorescent Protein-Based Multifunctional Affinity Purification Approach in Mammalian Cells
Hanhui Ma, Janel R. McLean, Kathleen L. Gould, and Dannel McCollum
15. Bimolecular Affinity Purification - A Variation of TAP with Multiple Applications
Petro Starokadomskyy and Ezra Burstein